Practical enzymic synthesis of adenosine 5'-0-(3-thiotriphosphate)(ATP-. gamma.-S)

O Abril, DC Crans, GM Whitesides

文献索引：Abril, Obsidiana; Crans, Debbie C.; Whitesides, George M. Journal of Organic Chemistry, 1984 , vol. 49, p. 1360 - 1364

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被引用次数: 17

摘要

An enzymatic procedure for the synthesis of adenosine 5'-0-(3-thiotriphosphate)(ATP-yS) on a 50-mmol scale from dihydroxyacetone, sodium thiophosphate, ADP, and phosphoenol pyruvate is described. The synthesis uses polyacrylamide gel immobilized glycerokinase coupled to a pyruvate kinase catalyzed ATP cofactor regeneration system, and polyacrylamide gel immobilized triceephcephate isomerase, glyceraldehyde 3-phosphate ...