An enzymatic procedure for the synthesis of adenosine 5'-0-(3-thiotriphosphate)(ATP-yS) on a 50-mmol scale from dihydroxyacetone, sodium thiophosphate, ADP, and phosphoenol pyruvate is described. The synthesis uses polyacrylamide gel immobilized glycerokinase coupled to a pyruvate kinase catalyzed ATP cofactor regeneration system, and polyacrylamide gel immobilized triceephcephate isomerase, glyceraldehyde 3-phosphate ...
