Polyphenols present in rooibos, a popular herbal tea from Aspalathus linearis, were isolated in two steps. First, phenolic ingredients were separated by multilayer countercurrent chromatography (MLCCC). Preparative high-performance liquid chromatography (HPLC) was then applied to obtain pure flavonoids. The purity and identity of isolated compounds was confirmed by different NMR experiments, HPLC-diode array detector (DAD), or gas ...
[Bell, Stephen G.; Zhou, Ruimin; Yang, Wen; Tan, Adrian B. H.; Gentleman, Alexander S.; Wong, Luet-Lok; Zhou, Weihong Chemistry - A European Journal, 2012 , vol. 18, # 52 p. 16677 - 16688]