Investigation of the substrate range of CYP199A4: modification of the partition between hydroxylation and desaturation activities by substrate and protein engineering

…, AS Gentleman, LL Wong, W Zhou

文献索引：Bell, Stephen G.; Zhou, Ruimin; Yang, Wen; Tan, Adrian B. H.; Gentleman, Alexander S.; Wong, Luet-Lok; Zhou, Weihong Chemistry - A European Journal, 2012 , vol. 18, # 52 p. 16677 - 16688

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被引用次数: 15

摘要

Abstract The cytochrome P450 enzyme CYP199A4, from Rhodopseudomonas palustris HaA2, can efficiently demethylate 4-methoxybenzoic acid. It is also capable of oxidising a range of other related substrates. By investigating substrates with different substituents and ring systems we have been able to show that the carboxylate group and the nature of the ring system and the substituent are all important for optimal substrate binding and activity. ...