![5-(((2,5-二氧吡咯烷-1-基)氧基)羰基)-3-羰基-3H-螺-[异苯并呋喃-1,9'-氧杂蒽]-3',6'-二基 二乙酸酯结构式](https://www.chemsrc.com/caspic/155/150206-05-6.png)
5-(((2,5-二氧吡咯烷-1-基)氧基)羰基)-3-羰基-3H-螺-[异苯并呋喃-1,9'-氧杂蒽]-3',6'-二基 二乙酸酯结构式
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常用名 | 5-(((2,5-二氧吡咯烷-1-基)氧基)羰基)-3-羰基-3H-螺-[异苯并呋喃-1,9'-氧杂蒽]-3',6'-二基 二乙酸酯 | 英文名 | 5-(((2,5-DIOXOPYRROLIDIN-1-YL)OXY)CARBONYL)-3-OXO-3H-SPIRO[ISOBENZOFURAN-1,9'-XANTHENE]-3',6'-DIYL DIACETATE |
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| CAS号 | 150206-05-6 | 分子量 | 557.461 | |
| 密度 | 1.6±0.1 g/cm3 | 沸点 | 757.9±70.0 °C at 760 mmHg | |
| 分子式 | C29H19NO11 | 熔点 | 152-154ºC(lit.) | |
| MSDS | 美版 | 闪点 | 412.2±35.7 °C |
用途5-羧基荧光素二乙酸酯N-琥珀酰亚胺酯是一种细胞渗透染料(Ex=492nm,Em=517nm)。5-羧基荧光素二乙酸酯N-琥珀酰亚胺酯可以通过与细胞内分子共价结合来标记细胞。5-羧基荧光素二乙酸酯N-琥珀酰亚胺酯用于跟踪淋巴细胞迁移和增殖[1]。 |
| 中文名 | 5-羧基二乙酰化荧光素琥珀酰亚胺酯 |
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| 英文名 | 5-Carboxy-fluorescein diacetate N-succinimidyl ester |
| 英文别名 | 更多 |
| 描述 | 5-羧基荧光素二乙酸酯N-琥珀酰亚胺酯是一种细胞渗透染料(Ex=492nm,Em=517nm)。5-羧基荧光素二乙酸酯N-琥珀酰亚胺酯可以通过与细胞内分子共价结合来标记细胞。5-羧基荧光素二乙酸酯N-琥珀酰亚胺酯用于跟踪淋巴细胞迁移和增殖[1]。 |
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| 相关类别 | |
| 体外研究 | 指南(以下是我们推荐的方案。本方案仅提供指南,应根据您的具体需要进行修改)[1]。1.在PBS中稀释储备溶液(得到50μM溶液)。2.每毫升细胞加入110μL该溶液(最终浓度为5μM),并快速混合。3.在室温下5分钟后,加入10体积的含有5%FBS的PBS。4.在300×g,20℃下离心细胞5min。5.除去上清液,洗涤三次,每次通过在含有5%FBS的10体积PBS中再悬浮。6.在300×g、20℃下离心细胞5分钟,并除去上清液。注意：标记发生得很快,跟踪器必须尽可能均匀、快速地分散,以便细胞被均匀标记。 |
| 参考文献 |
| 密度 | 1.6±0.1 g/cm3 |
|---|---|
| 沸点 | 757.9±70.0 °C at 760 mmHg |
| 熔点 | 152-154ºC(lit.) |
| 分子式 | C29H19NO11 |
| 分子量 | 557.461 |
| 闪点 | 412.2±35.7 °C |
| 精确质量 | 557.095825 |
| PSA | 151.81000 |
| LogP | 0.50 |
| 蒸汽压 | 0.0±2.6 mmHg at 25°C |
| 折射率 | 1.701 |
| 储存条件 | -20℃，惰性气体 |
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