BCECF

Modify Date: 2024-01-31 22:12:06

BCECF Structure
BCECF structure
Common Name BCECF
CAS Number 85138-49-4 Molecular Weight 520.44100
Density 1.67g/cm3 Boiling Point 936.2ºC at 760mmHg
Molecular Formula C27H20O11 Melting Point N/A
MSDS USA Flash Point 318.2ºC

 Use of BCECF


BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm.

 Names

Name 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
Synonym More Synonyms

 BCECF Biological Activity

Description BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm.
Related Catalog
In Vitro Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF[1].
Kinase Assay The cells are palced in a petri dish and BCECF present in trichomonads is fixed for electron microsopy. Controns without BCECF loading, without DAB treatment, and without UV illumination are run in parallel. Trichomonads remaine viable during the procedure, except that without BCECF they are dead and disrupted by 4 h. Then the cell are lysed. After lysis, BCECF is added to the lysate to 20 μM, and the lysate is incubated,followed by 3 washes in lysis buffer to remove free dye and resuspension of the pellet in the original volume[1].
References

[1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45.

 Chemical & Physical Properties

Density 1.67g/cm3
Boiling Point 936.2ºC at 760mmHg
Molecular Formula C27H20O11
Molecular Weight 520.44100
Flash Point 318.2ºC
Exact Mass 520.10100
PSA 187.89000
LogP 3.39840
Index of Refraction 1.743
Storage condition -20°C

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xn
Risk Phrases 20/21/22-36/37/38
Safety Phrases 22-24/25
RIDADR NONH for all modes of transport
WGK Germany 3.0

 Articles52

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Local pH domains regulate NHE3-mediated Na⁺ reabsorption in the renal proximal tubule.

Am. J. Physiol. Renal Physiol. 307(11) , F1249-62, (2014)

The proximal tubule Na(+)/H(+) exchanger 3 (NHE3), located in the apical dense microvilli (brush border), plays a major role in the reabsorption of NaCl and water in the renal proximal tubule. In resp...

Role of polyol moiety of amphotericin B in ion channel formation and sterol selectivity in bilayer membrane.

Bioorg. Med. Chem. 23 , 5782-8, (2015)

Amphotericin B (AmB) is a polyene macrolide antibiotic widely used to treat mycotic infections. In this paper, we focus on the role of the polyol moiety of AmB in sterol selectivity using 7-oxo-AmB, 7...

A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression.

Biochim. Biophys. Acta 1847 , 1254-66, (2015)

Abnormal accumulation of oncometabolite fumarate and succinate is associated with inhibition of mitochondrial function and carcinogenesis. By competing with α-ketoglutarate, oncometabolites also activ...

 Synonyms

2-[2,7-bis(2-carboxyethyl)-3-hydroxy-6-oxoxanthen-9-yl]terephthalic acid
BCECF
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