boc-val-pro-arg-amc hcl
Modify Date: 2025-08-24 11:54:34
boc-val-pro-arg-amc hcl structure
|
Common Name | boc-val-pro-arg-amc hcl | ||
|---|---|---|---|---|
| CAS Number | 70375-24-5 | Molecular Weight | 664.19300 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C31H46ClN7O7 | Melting Point | N/A | |
| MSDS | USA | Flash Point | N/A | |
Use of boc-val-pro-arg-amc hclBoc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1]. |
| Name | tert-butyl N-[1-[2-[[5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]carbamate,hydrochloride |
|---|---|
| Synonym | More Synonyms |
| Description | Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1]. |
|---|---|
| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 胰蛋白酶活性测定[2]1. 将稀释的 Trypsin 酶 (100 μL) 加入到 800 μL 的 50mM Tris-HCl 缓冲液 (pH 8.0) 中。 2. 加入 100 μL 50 μM 底物 Boc-Val-Pro-Arg-MCA 可立即引发反应。 3. 55°C 孵育 10 分钟。 4. 加入 1.5 mL 停止剂 (甲醇:正丁醇:蒸馏水= 35:30:35,v/v/v) 停止反应。 5. 用荧光光谱仪测量荧光强度 (excitation 380 nm, emission 450 nm)。 |
| References |
| Molecular Formula | C31H46ClN7O7 |
|---|---|
| Molecular Weight | 664.19300 |
| Exact Mass | 663.31500 |
| PSA | 208.95000 |
| LogP | 5.11390 |
| InChIKey | ROQGJTZGLVLVIN-AAJWHBHYSA-N |
| SMILES | Cc1cc(=O)oc2cc(NC(=O)C(CCCN=C(N)N)NC(=O)C3CCCN3C(=O)C(NC(=O)OC(C)(C)C)C(C)C)ccc12.Cl |
| Storage condition | −20°C |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
|
TFPI inhibits lectin pathway of complement activation by direct interaction with MASP-2.
Eur. J. Immunol. 45(2) , 544-50, (2015) The lectin pathway (LP) of complement has a protective function against invading pathogens. Recent studies have also shown that the LP plays an important role in ischemia/reperfusion (I/R)-injury. MBL... |
|
|
Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates.
Meth. Enzymol. 80 , 341, (1981)
|
|
|
New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.
J. Biochem. Tokyo 82 , 1495, (1977) Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides con... |
| Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride |
| MFCD00058181 |