boc-val-pro-arg-amc hcl

Names

[ Name ]:
boc-val-pro-arg-amc hcl

[Synonym ]:
Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride
MFCD00058181

Biological Activity

[Description]:

Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 胰蛋白酶活性测定[2]1. 将稀释的 Trypsin 酶 (100 μL) 加入到 800 μL 的 50mM Tris-HCl 缓冲液 (pH 8.0) 中。 2. 加入 100 μL 50 μM 底物 Boc-Val-Pro-Arg-MCA 可立即引发反应。 3. 55°C 孵育 10 分钟。 4. 加入 1.5 mL 停止剂 (甲醇:正丁醇:蒸馏水= 35:30:35,v/v/v) 停止反应。 5. 用荧光光谱仪测量荧光强度 (excitation 380 nm, emission 450 nm)。

[References]

[1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204.

[2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358.

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₁H₄₆ClN₇O₇

[ Molecular Weight ]:
664.19300

[ Exact Mass ]:
663.31500

[ PSA ]:
208.95000

[ LogP ]:
5.11390

[ Storage condition ]:
−20°C

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

TFPI inhibits lectin pathway of complement activation by direct interaction with MASP-2.

Eur. J. Immunol. 45(2) , 544-50, (2015)

The lectin pathway (LP) of complement has a protective function against invading pathogens. Recent studies have also shown that the LP plays an important role in ischemia/reperfusion (I/R)-injury. MBL...

Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates.

Meth. Enzymol. 80 , 341, (1981)

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

J. Biochem. Tokyo 82 , 1495, (1977)

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides con...

Related Compounds

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