Brilliant blue G-250

Modify Date: 2024-01-02 19:16:00

Brilliant blue G-250 structure	Common Name	Brilliant blue G-250
	CAS Number	6104-58-1	Molecular Weight	854.020
	Density	>1.0 g/cm3 (20ºC)	Boiling Point	N/A
	Molecular Formula	C₄₇H₄₈N₃NaO₇S₂	Melting Point	100 °C
	MSDS	USA	Flash Point	11 °C
	Symbol	GHS02, GHS06, GHS08	Signal Word	Danger

Use of Brilliant blue G-250

Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].

Name	Acid Blue 90
Synonym	More Synonyms

Description	Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
References	[1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3. [2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328. [3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.

Description

Related Catalog

Research Areas >> Others

Signaling Pathways >> Others >> Others

References

[1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3.

[2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328.

[3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.

Density	>1.0 g/cm3 (20ºC)
Melting Point	100 °C
Molecular Formula	C₄₇H₄₈N₃NaO₇S₂
Molecular Weight	854.020
Flash Point	11 °C
Exact Mass	853.283142
PSA	158.67000
LogP	11.19820
Storage condition	Store at RT.

Brilliant blue G-250 MSDS(Chinese)

Symbol	GHS02, GHS06, GHS08
Signal Word	Danger
Hazard Statements	H225-H301-H311-H315-H319-H331-H370
Precautionary Statements	P210-P260-P280-P301 + P310-P305 + P351 + P338-P311
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	F,T,Xn
Risk Phrases	R36/37/38
Safety Phrases	S22-S24/25-S36-S26-S45-S36/37/39-S16-S9-S7
RIDADR	UN 2924 3/PG 2
WGK Germany	3
HS Code	32041200

HS Code	32041200

Contrast recognizability during brilliant blue G - and heavier-than-water brilliant blue G-assisted chromovitrectomy: a quantitative analysis.

Acta Ophthalmol. 91(2) , e120-4, (2013)

To evaluate the potential of heavier-than-water brilliant blue G (BBG-D(2) 0) to stain the internal limiting membrane (ILM) during chromovitrectomy.In a nonrandomized, prospective, clinical multicentr...

Reconfiguring the quorum-sensing regulator SdiA of Escherichia coli to control biofilm formation via indole and N-acylhomoserine lactones.

Appl. Environ. Microbiol. 75 , 1703-16, (2009)

SdiA is a homolog of quorum-sensing regulators that detects N-acylhomoserine lactone (AHL) signals from other bacteria. Escherichia coli uses SdiA to reduce its biofilm formation in the presence of bo...

Lipopolysaccharide inhibits the channel activity of the P2X7 receptor.

Mediators Inflamm. 2011 , 152625, (2011)

The purinergic P2X7 receptor (P2X7R) plays an important role during the immune response, participating in several events such as cytokine release, apoptosis, and necrosis. The bacterial endotoxin lipo...

CBB G-250

benzenemethanaminium, N-[(1Z,4E)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)

Coomassie Brilliant Blue G250

Benzenemethanaminium, N-[(1E,4E)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)

EINECS 228-058-4

Cyanine G

benzenemethanaminium, N-[(1E,4Z)-4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-, inner salt, sodium salt (1:1)

PAGE BLUE G90

coomassie briliant blue G-250

coomassie blue G-250

Sodium 3-({[(1E,4Z)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methyl-2,5-cyclohexadien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate

Coomassie Brilliant blue

Polar Blue G

Blue G 250

BRILLIANT BLUE

Sodium 3-({[(1Z,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methylcyclohexa-2,5-dien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate

coomassie brillant blue G

SERVA BLUE G

MFCD00078482

Sodium 3-({[(1E,4E)-4-({4-[(4-ethoxyphenyl)amino]phenyl}{4-[ethyl(3-sulfonatobenzyl)amino]-2-methylphenyl}methylene)-3-methyl-2,5-cyclohexadien-1-ylidene](ethyl)ammonio}methyl)benzenesulfonate