Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester
Modify Date: 2024-01-06 12:04:55
![Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester Structure](https://www.chemsrc.com/caspic/349/1513848-14-0.png)
Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester structure
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Common Name | Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester | ||
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| CAS Number | 1513848-14-0 | Molecular Weight | 416.51 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C20H20N2O4S2 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl esterTRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm[1]. |
| Name | TRFS-green |
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| Description | TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm[1]. |
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| Related Catalog | |
| In Vitro | TRFS-green itself emits at ∼480 nm when excited at 377 nm[1]. TRFS-green displays a green fluorescence off−on change induced by the TrxR-mediated disulfide cleavage and subsequent intramolecular cyclization to liberate the masked naphthalimide fluorophore[1]. The fluorescence signal of TRFS-green in living cells was inhibited by pretreating the cells with TrxR inhibitor in a dose-dependent manner, potentiating the development of living cell-based screening assay for identifying TrxR inhibitors[1]. After incubating the cells with TRFS-green (10 μM) for 4 h, the bright green fluorescence appears[1]. TRFS-green shows no response to GSH or Cys, the predominant thiols in cells, or the endogenous reducing agents, such as vitamin C and NADPH[1]. TRFS-green is the first fluorescent probe of TrxR with a green emission. However, the response rate of TRFS-green to the enzyme is slow, and it takes more than 2 h to reach the maximal fluorescence signal even using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent[2]. |
| References |
| Molecular Formula | C20H20N2O4S2 |
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| Molecular Weight | 416.51 |