1513848-14-0
1513848-14-0 structure
- Name: Carbamic acid, N-(2-butyl-2,3-dihydro-1,3-dioxo-1H-benz[de]isoquinolin-6-yl)-, 1,2-dithiolan-4-yl ester
- Chemical Name: TRFS-green
- CAS Number: 1513848-14-0
- Molecular Formula: C20H20N2O4S2
- Molecular Weight: 416.51
- Catalog: Research Areas Others
- Create Date: 2021-09-11 12:09:34
- Modify Date: 2024-01-06 12:04:55
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TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm[1].
| Name | TRFS-green |
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| Description | TRFS-green is a highly selective off−on fluorescent probe for imaging thioredoxin reductase (TrxR) in living cells. TRFS-green has the maximum absorbance at around 373 nm[1]. |
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| Related Catalog | |
| In Vitro | TRFS-green itself emits at ∼480 nm when excited at 377 nm[1]. TRFS-green displays a green fluorescence off−on change induced by the TrxR-mediated disulfide cleavage and subsequent intramolecular cyclization to liberate the masked naphthalimide fluorophore[1]. The fluorescence signal of TRFS-green in living cells was inhibited by pretreating the cells with TrxR inhibitor in a dose-dependent manner, potentiating the development of living cell-based screening assay for identifying TrxR inhibitors[1]. After incubating the cells with TRFS-green (10 μM) for 4 h, the bright green fluorescence appears[1]. TRFS-green shows no response to GSH or Cys, the predominant thiols in cells, or the endogenous reducing agents, such as vitamin C and NADPH[1]. TRFS-green is the first fluorescent probe of TrxR with a green emission. However, the response rate of TRFS-green to the enzyme is slow, and it takes more than 2 h to reach the maximal fluorescence signal even using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent[2]. |
| References |
| Molecular Formula | C20H20N2O4S2 |
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| Molecular Weight | 416.51 |