Flavopiridol HCl
Modify Date: 2024-01-09 21:35:00
Flavopiridol HCl structure
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Common Name | Flavopiridol HCl | ||
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| CAS Number | 131740-09-5 | Molecular Weight | 438.301 | |
| Density | N/A | Boiling Point | 603.6ºC at 760 mmHg | |
| Molecular Formula | C21H21Cl2NO5 | Melting Point | 169.5-170ºC | |
| MSDS | Chinese | Flash Point | 318.8ºC | |
Use of Flavopiridol HClFlavopiridol Hydrochloride is a broad inhibitor of CDK, competing with ATP to inhibit CDKs including CDK1, CDK2, CDK4 with IC50s of 30, 170, 100 nM, respectively. |
| Name | 2-(2-chlorophenyl)-5,7-dihydroxy-8-[(3S,4R)-3-hydroxy-1-methylpiperidin-4-yl]chromen-4-one,hydrochloride |
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| Synonym | More Synonyms |
| Description | Flavopiridol Hydrochloride is a broad inhibitor of CDK, competing with ATP to inhibit CDKs including CDK1, CDK2, CDK4 with IC50s of 30, 170, 100 nM, respectively. |
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| Related Catalog | |
| Target |
CDK1/Cyc B1:30 nM (IC50) CDK2/Cyc E:170 nM (IC50) CDK4/Cyc D1:100 nM (IC50) MAP:19000 nM (IC50) PKC:14000 nM (IC50) EGFR:22000 nM (IC50) |
| In Vitro | Flavopiridol (2 μM) robustly induces a distinct pattern of ER stress in CLL cells that contributes to cell death through IRE1-mediated activation of ASK1 and possibly downstream caspases[1]. Flavopiridol results in potent upregulation of a number of PRGs in treatments lasting 4-24 h. Flavopiridol has and immediate and long-term effect on the expression of several PRGs. In serum starved cells re-stimulated with serum, flavopiridol also inhibits the expression of these genes, but subsequently, JUNB, GADD45B and EGR1 are upregulated in the presence of flavopiridol[2]. |
| Kinase Assay | Briefly, lysates containing approximately 3×106 cells are incubated with 50 μM LEVD-AFC (caspase 4 substrate) or LETD-AFC (caspase 8 substrate) containing 10 mM dithiothretiol (DTT). Caspase 4 activity is measured one hour after addition of substrate and caspase 8 activity is measured 30 minutes after addition of substrate. Release of free AFC is measured with a Beckman-Coulter DTX 880 multimode detector. |
| Cell Assay | The cells treated with flavopiridol are washed after 4 hours with PBS and resuspended in regular growth medium (RPMI 1640) supplemented with 10% human serum and antibiotics for the remainder of the incubation time. In the case of flavopiridol/chloroquine samples, chloroquine is re-added in the fresh media after flavopiridol is washed at 4 hours. For all the other conditions, cells are incubated with the respective drugs for 24 hours continuously. |
| References |
| Boiling Point | 603.6ºC at 760 mmHg |
|---|---|
| Melting Point | 169.5-170ºC |
| Molecular Formula | C21H21Cl2NO5 |
| Molecular Weight | 438.301 |
| Flash Point | 318.8ºC |
| Exact Mass | 437.079681 |
| PSA | 94.14000 |
| LogP | 4.04450 |
| Vapour Pressure | 2.03E-15mmHg at 25°C |
| Storage condition | -20℃ |
| Hazard Codes | Xn: Harmful; |
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| Risk Phrases | R22 |
| RTECS | DJ2978830 |
| Alvocidib hydrochloride |
| HL-275 |
| 4H-1-Benzopyran-4-one, 2-(2-chlorophenyl)-5,7-dihydroxy-8-[(3S,4R)-3-hydroxy-1-methyl-4-piperidinyl]-, hydrochloride (1:1) |
| 2-(2-Chlorophenyl)-5,7-dihydroxy-8-[(3S,4R)-3-hydroxy-1-methyl-4-piperidinyl]-4H-chromen-4-one hydrochloride (1:1) |
| (-)-2-(2-Chlorophenyl)-5,7-dihydroxy-8-[(3s,4r)-3-hydroxy-1-methyl-4-piperidinyl]-4h-1-benzopyran-4-one hydrochloride |
| Flavopiridol HCl |
| Flavopiridol (Alvocidib) HCl |
| Flavopiridol (Hydrochloride) |
| HMR-1275 |
| Flavopiridol Hydrochloride |
| L 86-8275 |
| Flavopiridol HCI |