Methyl-β-cyclodextrin

Modify Date: 2024-01-02 10:12:39

Methyl-β-cyclodextrin structure	Common Name	Methyl-β-cyclodextrin
	CAS Number	128446-36-6	Molecular Weight	1303.303
	Density	1.4±0.1 g/cm3	Boiling Point	1206.9±65.0 °C at 760 mmHg
	Molecular Formula	C₅₄H₉₄O₃₅	Melting Point	180-182ºC
	MSDS	USA	Flash Point	683.7±34.3 °C

Use of Methyl-β-cyclodextrin

Methyl-β-cyclodextrin, a cyclic heptasaccharide, is an effective agent for the depletion of cholesterol from cells; also inhibits PEL cell growth with an IC50 of 3.33-4.23 mM.

Name	beta-Cyclodextrin methyl ethers
Synonym	More Synonyms

Description	Methyl-β-cyclodextrin, a cyclic heptasaccharide, is an effective agent for the depletion of cholesterol from cells; also inhibits PEL cell growth with an IC50 of 3.33-4.23 mM.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Cancer Biochemical Assay Reagents
Target	IC50: 3.33-4.23 mM (PEL cell growth)[1]
In Vitro	Cyclodextrins are a family of cyclic oligosaccharides with a hydrophilic outer surface and a lipophilic central cavity. Cyclodextrins molecules are relatively large with a number of hydrogen donors and acceptors and, thus in general, they do not permeate lipophilic membranes. In the pharmaceutical industry, cyclodextrins have mainly been used as complexing agents to increase aqueous solubility of poorly soluble drugs and to increase their bioavailability and stability. Cyclodextrins are used in pharmaceutical applications for numerous purposes, including improving the bioavailability of drugs[2]. Methyl-β-cyclodextrin quickly induces caspase-dependent apoptosis in PEL cells via cholesterol depletion from the plasma membrane. Methyl-β-cyclodextrin inhibits the growth of all PEL cell lines in a dose-dependent manner. The IC50 is 3.33-4.23 mM in each cell line[1]. Methyl-b-cyclodextrin is a highly water soluble cyclic heptasaccharide consisting of a β-glucopyranose unit, has been reported as the most effective agent for the depletion of cholesterol from cells among the various cholesterol-depleting agents[1].
In Vivo	In a PEL xenograft mouse model, methyl-b-cyclodextrin significantly inhibits the growth and invasion of PEL cells without apparent adverse effects. Methyl-b-cyclodextrin-treated mice appears to be healthy, whereas non-treated mice has a distended abdominal region. The body weights of control are significantly higher than those of M-b-CyD treated mice. Methyl-b-cyclodextrin-treated mice has a significantly lower volume of ascites than that of non-treated mice[1]. Studies in both humans and animals have shown that cyclodextrins can be used to improve drug delivery from almost any type of drug formulation. Currently, there are approximately 30 different pharmaceutical products worldwide containing drug/cyclodextrins complexes in the market[2].
Cell Assay	PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1].
Animal Admin	Mice: Female NRJ mice are intraperitoneally inoculated with BCBL-1 cells suspended in PBS. The mice are then treated with intraperitoneal injections of PBS or methyl-β-cyclodextrin (500 mg/kg per day). Tumor burdens are evaluated by measuring body weights and ascites[1].
References	[1]. Gotoh K, et al. The antitumor effects of methyl-β-cyclodextrin against primary effusion lymphoma via the depletion of cholesterol from lipid rafts. Biochem Biophys Res Commun. 2014 Dec 12;455(3-4):285-9. [2]. Tiwari G, et al. Cyclodextrins in delivery systems: Applications. J Pharm Bioallied Sci. 2010 Apr;2(2):72-9.

Density	1.4±0.1 g/cm3
Boiling Point	1206.9±65.0 °C at 760 mmHg
Melting Point	180-182ºC
Molecular Formula	C₅₄H₉₄O₃₅
Molecular Weight	1303.303
Flash Point	683.7±34.3 °C
Exact Mass	1302.557617
PSA	422.05000
LogP	6.95
Vapour Pressure	0.0±0.6 mmHg at 25°C
Index of Refraction	1.567
Storage condition	2~8°C
Water Solubility	H2O: soluble50mg/mL \| Soluble in water

Methyl-β-cyclodextrin MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	T+
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3

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Comparative study on inclusion complexation of maltosyl-beta-cyclodextrin, heptakis(2,6-di-O-methyl)-beta-cyclodextrin and beta-cyclodextrin with fucosterol in aqueous and solid state.

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The complexation of fucosterol with three kinds of beta-cyclodextrin (beta-CyD) was investigated in aqueous solution and in the solid state. The solubility of fucosterol increased significantly on its...

β-Cyclodextrin methyl ethers

Methyl-Beta-Cyclodextrin

Methyl-β-cyclodextrin

MFCD00074980

(1S,3R,5R,6R,8R,10R,11R,13R,15R,16R,18R,20R,21R,23R,25R,26R,28R,30R,31S,33R,35R,36R,37S,38R,39S,40R,41S,42R,43S,44R,45S,46R,47S,48R,49S)-5,10,15,20,25,30,35-Heptakis(hydroxymethyl)-37,39,40,41,42,43,4 ;4,45,46,47,48,49-dodecamethoxy-2,4,7,9,12,14,17,19,22,24,27,29,32,34-tetradecaoxaoctacyclo[31.2.2.2.2.2.2.2.2]nonatetracontane-36,38-diol

Randomly methylated-β-Cyclodextrin

Methyl-beta-cyclodextrin,average Mn ~1310