WAY-600
Modify Date: 2024-01-02 19:32:38
WAY-600 structure
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Common Name | WAY-600 | ||
|---|---|---|---|---|
| CAS Number | 1062159-35-6 | Molecular Weight | 494.591 | |
| Density | 1.4±0.1 g/cm3 | Boiling Point | 678.1±55.0 °C at 760 mmHg | |
| Molecular Formula | C28H30N8O | Melting Point | N/A | |
| MSDS | N/A | Flash Point | 363.9±31.5 °C | |
Use of WAY-600WAY-600 is a potent, ATP-competitive, and selective mTOR inhibitor with an IC50 of 9 nM for recombinant mTOR enzyme. WAY-600 blocks mTOR complex 1/2 (mTORC1/2) assemble and activation. |
| Name | 4-[6-(1H-indol-5-yl)-1-[1-(pyridin-3-ylmethyl)piperidin-4-yl]pyrazolo[3,4-d]pyrimidin-4-yl]morpholine |
|---|---|
| Synonym | More Synonyms |
| Description | WAY-600 is a potent, ATP-competitive, and selective mTOR inhibitor with an IC50 of 9 nM for recombinant mTOR enzyme. WAY-600 blocks mTOR complex 1/2 (mTORC1/2) assemble and activation. |
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| Related Catalog | |
| Target |
mTOR:9 nM (IC50) mTORC1 mTORC2 PI3K alpha:1.96 μM (IC50) PI3K gamma:8.45 μM (IC50) |
| In Vitro | WAY-600 exhibits a concentration-dependent and time-dependent inhibition of f HepG2 and Huh-7 cells viability. Following WAY-600 (1-1000 nM) treatment, the number of HepG2 cell colonies is dramatically decreased. Meanwhile, BrdU incorporation in HepG2 cells is also inhibited with WAY-600 treatment. WAY-600 dose-dependently increases the activity of caspase-3 and caspase-9 in HepG2 cells. WAY-600 disrupts assemble of mTORC1 (mTOR-Raptor association) and mTORC2 (mTOR-Rictor association). Activation of mTORC1 (indicated by p-S6K1 and p-4E-BP1) and mTORC2 is almost blocked by WAY-600 (100 nM)[2]. |
| In Vivo | Administration of WAY-600 (10 mg/kg, daily) inhibits HepG2 tumor growth in nude mice. Daily HepG2 tumor growth of WAY-600-administrated mice is significantly lower than that of vehicle control mice. Importantly, the in vivo anti-cancer activity by WAY-600 is further potentiated with the co-administration of MEK-162 (2.5 mg/kg, p.o. daily)[2]. |
| Cell Assay | Established HCC cells (HepG2 and Huh-7), primary HCC cells (Pnt-1/-2/-3/-4), or THLE-2 liver cells are cultured in WAY-600 (1-1000 nM)-containing medium for 24, 48, 72, 96 hours, cell viability is tested by MTT assay[2]. |
| Animal Admin | Mice: Mice tumor xenografts are randomly divided into four groups (10 mice per group): vehicle ( intraperitoneal or i.p.), WAY-600 (10 mg/kg, i.p. injection), MEK-162 (2.5 mg/kg, oral gavage) or WAY-600 plus MEK-162 combination. The mice are monitored for activity and physical condition on daily basis, and mice body weights and tumor mass are measured weekly[2]. |
| References |
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 678.1±55.0 °C at 760 mmHg |
| Molecular Formula | C28H30N8O |
| Molecular Weight | 494.591 |
| Flash Point | 363.9±31.5 °C |
| Exact Mass | 494.254272 |
| PSA | 87.99000 |
| LogP | 1.30 |
| Vapour Pressure | 0.0±2.1 mmHg at 25°C |
| Index of Refraction | 1.761 |
| Storage condition | -20℃ |
| 6-(1H-Indol-5-yl)-4-(4-morpholinyl)-1-[1-(3-pyridinylmethyl)-4-piperidinyl]-1H-pyrazolo[3,4-d]pyrimidine |
| X7456 |
| 1H-Pyrazolo[3,4-d]pyrimidine, 6-(1H-indol-5-yl)-4-(4-morpholinyl)-1-[1-(3-pyridinylmethyl)-4-piperidinyl]- |
| WAY-600 |
| pyrazolo pyrimidine,5u |