1224844-38-5
1224844-38-5 structure
- Name: Sapanisertib (MLN0128)
- Chemical Name: 5-(4-amino-1-propan-2-ylpyrazolo[3,4-d]pyrimidin-3-yl)-1,3-benzoxazol-2-amine
- CAS Number: 1224844-38-5
- Molecular Formula: C15H15N7O
- Molecular Weight: 309.326
- Catalog: Biochemical Inhibitor PI3K/Akt/mTOR inhibitor (PI3K/Akt/mTOR) mTOR inhibitor
- Create Date: 2018-06-02 08:00:00
- Modify Date: 2024-01-02 11:22:42
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Sapanisertib (INK-128) is a ATP-dependent mTOR1/2 inhibitor with an IC50 of 1 nM for mTOR kinase.
| Name | 5-(4-amino-1-propan-2-ylpyrazolo[3,4-d]pyrimidin-3-yl)-1,3-benzoxazol-2-amine |
|---|---|
| Synonyms |
cs-0557
2-Benzoxazolamine, 5-[4-amino-1-(1-methylethyl)-1H-pyrazolo[3,4-d]pyrimidin-3-yl]- UNII-JGH0DF1U03 3-(2-Amino-1,3-benzoxazol-5-yl)-1-isopropyl-1H-pyrazolo[3,4-d]pyrimidin-4-amine Sapanisertib ink-128/ink128 MLN-0128 ink 128 UNII:JGH0DF1U03 INK-128 |
| Description | Sapanisertib (INK-128) is a ATP-dependent mTOR1/2 inhibitor with an IC50 of 1 nM for mTOR kinase. |
|---|---|
| Related Catalog | |
| Target |
mTOR:1 nM (IC50) mTORC1 mTORC2 PI3Kα:219 nM (IC50) PI3Kβ:5.293 μM (IC50) PI3Kδ:230 nM (IC50) PI3Kγ:221 nM (IC50) Autophagy |
| In Vitro | Sapanisertib (INK-128) exhibits an enzymatic inhibition activity against mTOR and more than 100-fold selectivity to PI3K kinases[1]. Sapanisertib (INK-128) selectively decreases the expression of YB1, MTA1, vimentin and CD44 at the protein but not transcript level in PC3 cells. Sapanisertib (INK-128) decreases the invasive potential of PC3 prostate cancer cells. Furthermore, Sapanisertib (INK-128) inhibits cancer cell migration starting at 6 h of treatment, precisely correlating with when decreases in the expression of pro-invasion genes are evident, but preceding any changes in the cell cycle or overall global protein synthesis[2]. |
| In Vivo | In a ZR-75-1 breast cancer xenograft model, Sapanisertib (INK-128) shows tumor growth inhibition efficacy at a dose of 0.3 mg/kg/day[1]. 4EBP1 and p70S6K1/2 phosphorylation is completely restored to wild-type levels after treatment with INK128 in PtenL/L mice. Sapanisertib (INK-128) treatment results in a 50% decrease in prostatic intraepithelial neoplasia (PIN) lesions in PtenL/L mice and induces programmed cell death in multiple cancer cell lines in mice[2]. |
| Cell Assay | PC3 cells are treated with the appropriate drug for 48 h, and proliferation is measured using CellTiter-Glo Luminescent reagent. The concentration of Sapanisertib (INK-128) necessary to achieve inhibition of cell growth by 50% (IC50) is calculated using concentrations ranging from 20.0 μM to 0.1 nM (12-point curve). |
| Animal Admin | Nude mice are inoculated subcutaneously in the right subscapular region with 5×106 MDA-MB-361 cells. After tumours reach a size of 150-200 mm3, mice are randomLy assigned into vehicle control or treatment groups. Sapanisertib (INK-128) is formulated in 5% polyvinylpropyline, 15% NMP, 80% water and administered by oral gavage at 0.3 mg/kg and 1 mg/kg daily. |
| References |
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 598.8±60.0 °C at 760 mmHg |
| Molecular Formula | C15H15N7O |
| Molecular Weight | 309.326 |
| Flash Point | 315.9±32.9 °C |
| Exact Mass | 309.133820 |
| PSA | 121.67000 |
| LogP | 1.95 |
| Vapour Pressure | 0.0±1.7 mmHg at 25°C |
| Index of Refraction | 1.829 |
| Storage condition | -20°C |
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1224844-38-5 |
| Literature: WO2013/23184 A1, ; WO 2013/023184 A1 |
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1224844-38-5 |
| Literature: WO2013/23184 A1, ; WO 2013/023184 A1 |
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1224844-38-5 |
| Literature: WO2013/23184 A1, ; WO 2013/023184 A1 |
| Precursor 3 | |
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| DownStream 0 | |