Name | 5-carboxyfluorescein |
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Synonyms |
MFCD00151081
3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-dihydroxy-3-oxo- EINECS 276-331-1 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid 5-carboxyfluorescein 5-FAM |
Description | The single isomer, 5-FAM, contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. |
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Related Catalog |
Density | 1.7±0.1 g/cm3 |
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Boiling Point | 725.2±60.0 °C at 760 mmHg |
Melting Point | 368-372°C |
Molecular Formula | C21H12O7 |
Molecular Weight | 376.316 |
Flash Point | 266.9±26.4 °C |
Exact Mass | 376.058289 |
PSA | 113.29000 |
LogP | 2.62 |
Vapour Pressure | 0.0±2.5 mmHg at 25°C |
Index of Refraction | 1.816 |
Storage condition | Store at 2-8 |
Water Solubility | DMF: soluble |
Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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Hazard Codes | Xi: Irritant; |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
HS Code | 2932999099 |
~% 76823-03-5 |
Literature: Russian Journal of General Chemistry, , vol. 65, # 3.2 p. 454 - 456 Zhurnal Obshchei Khimii, , vol. 65, # 3 p. 511 - 513 |
~% 76823-03-5 |
Literature: Chemical communications (Cambridge, England), , # 23 p. 2870 - 2871 |
~% 76823-03-5 |
Literature: Journal of the American Chemical Society, , vol. 135, # 47 p. 17659 - 17662 |
~% 76823-03-5 |
Literature: Chemische Berichte, , vol. 11, p. 1342 |
Precursor 3 | |
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DownStream 1 | |
HS Code | 2932999099 |
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Summary | 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |