95378-73-7

95378-73-7 structure

Name: 4-Di-10-ASP
Chemical Name: 4-(4-(Didecylamino)styryl)-N-methylpyridinium iodide,4-Di-10-ASP
CAS Number: 95378-73-7
Molecular Formula: C₃₄H₅₅IN₂
Molecular Weight: 618.71800
Catalog: Research Areas Others
Create Date: 2018-12-25 21:00:03
Modify Date: 2024-01-30 11:09:43
4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner[1][2].

Name	4-(4-(Didecylamino)styryl)-N-methylpyridinium iodide,4-Di-10-ASP
Synonyms	4-di-10-asp iodide 4-di-10-asp

Description	4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. 4-Di-10-ASP (1 μM), DOPC (HY-113424A) (10 mM), and DOPG (HY-142980) (1 mM) is dissolved in methanol/chloroform (25 L, 1:2 v/v). 2. The solution is allowed to dry overnight under vacuum to obtain lamellar lipid films, which in turn are hydrated with the transcription/translation solution (25 L) for three hours at 37℃. 3. An aliquot (10 L) of the solution thus prepared is placed on a glass slide and sealed by a cover glass. 4. The sample is immediately observed with a confocal laser-scanning microscope, an argon laser (488 nm) is employed to excite the 4-Di-10-ASP[2].
References	[1]. Z J Huang, et al. Partition coefficients of fluorescent probes with phospholipid membranes. Biochem Biophys Res Commun. 1991 Nov 27;181(1):166-71. [2]. Shin-ichiro M Nomura, et al. Gene expression within cell-sized lipid vesicles. Chembiochem. 2003 Nov 7;4(11):1172-5.