86636-92-2
86636-92-2 structure
- Name: Acrylodan
- Chemical Name: 1-[6-(dimethylamino)naphthalen-2-yl]prop-2-en-1-one
- CAS Number: 86636-92-2
- Molecular Formula: C15H15NO
- Molecular Weight: 225.28600
- Catalog: Research Areas Others
- Create Date: 2018-03-11 08:00:00
- Modify Date: 2025-08-23 19:40:04
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Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34[1][2].
| Name | 1-[6-(dimethylamino)naphthalen-2-yl]prop-2-en-1-one |
|---|---|
| Synonyms |
Acrylodan
6-Acryloyl-2-dimethylaminonaphthalene |
| Description | Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34[1][2]. |
|---|---|
| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Purification and Labeling of Act1: 1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight. 2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C. 3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M−1 cm−1 for actin at 290 nm, 18,500 M−1 cm−1 or acrylodan at 385 nm, and 71,000 M−1 cm−1 for Alexa Fluor 488 at 494 nm. 4. Acrylodan-labeled Act1 (AcrylAct1NAT) is dramatically different than that of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1. |
| References |
| Density | 1.1g/cm3 |
|---|---|
| Boiling Point | 388.3ºC at 760mmHg |
| Molecular Formula | C15H15NO |
| Molecular Weight | 225.28600 |
| Flash Point | 153.1ºC |
| Exact Mass | 225.11500 |
| PSA | 20.31000 |
| LogP | 3.27450 |
| Index of Refraction | 1.63 |
| Hazard Codes | Xi |
|---|---|
| HS Code | 2922399090 |
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86636-92-2 |
| Literature: Organic Preparations and Procedures International, , vol. 37, # 6 p. 589 - 594 |
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86636-92-2 |
| Literature: Organic Preparations and Procedures International, , vol. 37, # 6 p. 589 - 594 |
|
~%
86636-92-2 |
| Literature: Organic Preparations and Procedures International, , vol. 37, # 6 p. 589 - 594 |
| Precursor 1 | |
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| DownStream 0 | |
| HS Code | 2922399090 |
|---|---|
| Summary | 2922399090 other amino-aldehydes, amino-ketones and amino-quinones, other than those containing more than one kind of oxygen function; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:30.0% |