219137-97-0

219137-97-0 structure

Name: Ac-Val-Glu-Ile-Asp-AMC
Chemical Name: ac-veid-amc
CAS Number: 219137-97-0
Molecular Formula: C₃₂H₄₃N₅O₁₁
Molecular Weight: 673.711
Catalog: Biochemical Amino acids and their derivatives Aspartic acid derivative
Create Date: 2018-12-31 16:19:14
Modify Date: 2024-01-08 16:24:52
Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].

Name	ac-veid-amc
Synonyms	L-α-Asparagine, N-acetyl-L-valyl-L-α-glutamyl-L-isoleucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- Ac-Val-Glu-IIe-Asp-7-amino-4-methylcoumarin N-Acetyl-L-valyl-L-α-glutamyl-L-isoleucyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)-L-α-asparagine AC-VAL-GLU-ILE-ASP-MCA AC-VEID-MCA PASE-6 SUBSTRATE I,FLUOROGENIC AC-VAL-GLU-ILE-ASP-AMC N-ACETYL-VAL-GLU-ILE-ASP-7-AMINO-4-METHYLCOUMARIN AMC AC-VAL-GLU-ILE-ASP-7-AMINO-4-METHYLCOUMARIN

Description	Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Enzyme activity (rabbit osteoclasts for example)[1]: 1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell). 2. Put treated cells into 4.5 mL lysis buffer. 3. Each lysate is divided into thirds, then incubate with 40 µM Ac-VEID-AMC at 37°C for 1 h. 4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)