62796-23-0

62796-23-0 structure

Name: 3(2H)-Benzoxazolepropanesulfonicacid,2-[4-(1,3-dibutyltetrahydro-4,6-dioxo-2-thioxo-5(2H)-pyrimidinylidene)-2-buten-1-ylidene]-,sodium salt (1:1)
Chemical Name: merocyanine 540
CAS Number: 62796-23-0
Molecular Formula: C₂₆H₃₂N₃NaO₆S₂
Molecular Weight: 569.66900
Catalog: Research Areas Others
Create Date: 2018-05-23 08:00:00
Modify Date: 2024-04-03 18:22:08
Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm)[1][2].

Name	merocyanine 540
Synonyms	MEROCYANINE 540 2-[4-(1,3-Dibutyl-1,3,4,6-tetrahydro-4,6-dioxo-2-thioxopyrimidin-5(2H)-ylidene)-2-butenylidene]-3(2H)-benzoxazolepropane-1-sulfonic acid sodium salt MFCD00013438 2-(4-(1,3-dibutyltetrahydro-4,6-dioxo-2-3(2h)-benzoxazolepropanesulfonicaci Merocyanin 540

Description	Merocyanin 540 is a fluorescent membrane probe that selectively stains the membranes of a wide variety of electrically excitable cells, but not those of nonexcitable cells (Ex/Em: 540/580 nm)[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines[1] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Wash monolayer cultured cells with isotonic saline with or without 1-5 mM CaCl2 or iso-osmotic sucrose (0.25 M) solutions buffered with 5 mM Tris-Cl (pH 7.4). 2. Expose cells to Merocyanin 540 (1.6 x 10-5 M) in the appropriate buffer for 10 min at room temperature under ambient lighting conditions. 3. Wash extensively and examine by fluorescence microscopy. 4. For protozoa, place 0.05 mL aliquots of washed cultures containing 100-200 organisms on a microscope slide. 5. Add Merocyanin 540 to a final concentration of 1.0-10 μg/mL. 6. Examine cells by fluorescence microscopy. 7. Incubate single cell suspensions or tissue slices with Merocyanin 540 (1.75-5.25 x 10-5 M) and examine by fluorescence microscopy as described for attached cells.
References	[1]. Easton TG, et al. Merocyanine 540 as a fluorescent probe of membranes: staining of electrically excitable cells. Cell. 1978 Mar;13(3):475-86. [2]. Lagerberg JW, et al. Factors affecting the amount and the mode of merocyanine 540 binding to the membrane of human erythrocytes. A comparison with the binding to leukemia cells. Biochim Biophys Acta. 1995 May 4;1235(2):428-36.

Melting Point	285ºC (dec.)(lit.)
Molecular Formula	C₂₆H₃₂N₃NaO₆S₂
Molecular Weight	569.66900
Exact Mass	569.16300
PSA	156.36000
LogP	4.22630
Storage condition	2-8°C

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DM4866500

CHEMICAL NAME :: 3(2H)-Benzoxazolepropanesulfonic acid, 2-(4-(1,3-dibutyltetrahydro-4,6-dioxo-2-thioxo-5(2H)- pyrimidinylidene)-2-butenylidene)-, sodium salt

CAS REGISTRY NUMBER :: 62796-23-0

LAST UPDATED :: 199706

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C26-H32-N3-O6-S2.Na

MOLECULAR WEIGHT :: 569.72

WISWESSER LINE NOTATION :: T56 BNYOJ B3SWO CU2U2U- ET6NYNVYVJ A4 BUS C4 &-NA-

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 84 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: USXXAM United States Patent Document. (U.S. Patent Office, Box 9, Washington, DC 20231) Volume(issue)/page/year: #4775625

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi
Risk Phrases	36/37/38
Safety Phrases	S26;S36
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	DM4866500

56-41-7	108-65-6
56-35-9	64-17-5
67-56-1	108-88-3
141-78-6	1310-73-2
13463-67-7	1333-86-4