41830-80-2

41830-80-2 structure

41830-80-2 structure

Name: Cresyl Violet perchlorate
Chemical Name: cresyl violet perchlorate
CAS Number: 41830-80-2
Molecular Formula: C₁₆H₁₂ClN₃O₅
Molecular Weight: 361.73700
Catalog: Dye Reagents
Create Date: 2018-08-22 11:11:13
Modify Date: 2024-01-10 21:38:12
Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

Name	cresyl violet perchlorate
Synonyms	CRESYL VIOLET PERCHLORATE Cresyl Violet 670 CRESYL VIOLET 670 PERCHLORATE) EINECS 255-561-6 Oxazine 9 perchlorate Cresyl Violet perchlorate,laser grade,pure Cresyl Violet perchlorate,laser grade Cresylvioletperchlorate,lasergrade MFCD00012671 Cresyl Violet perchlorate,pure,laser grade

Description	Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Neurological Disease
In Vitro	The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1].
Cell Assay	Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].
References	[1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.

Boiling Point	482.3ºC at 760mmHg
Melting Point	331 - 333ºC
Molecular Formula	C₁₆H₁₂ClN₃O₅
Molecular Weight	361.73700
Flash Point	245.5ºC
Exact Mass	361.04700
PSA	147.34000
LogP	3.98470
Index of Refraction	1.758

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi
Risk Phrases	R36/37/38
Safety Phrases	S26-S36
RIDADR	NONH for all modes of transport
WGK Germany	3