Name | LM-1484 |
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Synonyms |
8-{(E)-2-[4-(4-Phenylbutoxy)phenyl]vinyl}-2-(2H-tetrazol-5-yl)-4H-chromen-4-one
4H-1-Benzopyran-4-one, 8-[(E)-2-[4-(4-phenylbutoxy)phenyl]ethenyl]-2-(2H-tetrazol-5-yl)- |
Description | LM-1484 is an antagonist of CysLT1 receptor and displays a higher affinity for 3H-LTC4 sites. |
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Related Catalog | |
Target |
CysLT1 LTC4 |
In Vitro | LM-1484 (10 μM) induces the dissociation of 3H-LTD4, and is able to displace 3H-LTC4 from its binding sites[1]. |
Kinase Assay | Equilibrium binding studies are performed at 25°C for 60 min with 0.03-0.5 nM 3H-LTD4 or 40 min with 0.03-0.5 nM 3H-LTC4 and unlabeled homologous or heterologous ligands at the indicated concentrations. A multiligand protocol is followed. Ten micromolar S-decyl-GSH is present only in the case of 3H-LTC4 equilibrium experiments. Time-courses are performed at 25°C with 0.5 nM 3H-LTC4 or 3H-LTD4. Dissociation is induced by adding 1 mM unlabeled leukotriene (homologous dissociation) or 10 μM unlabeled antagonist (heterologous dissociation). In both equilibrium and kinetic studies HLP membranes (0.25 mg per sample), 10 mM HEPES-KOH pH 7.4, 1 mM CaCl2 and 1 mM MgCl2 are added to the incubation mixture to achieve a final volume of 250 mL. All the experiments have been performed under control metabolic conditions. Unbound ligand is separated by rapid vacuum filtration onto glass-fiber GF/C filters soaked in 2.5% polyvinylalchool and the filters are washed twice with 4 mL of HEPES buffer at 4°C. Radioactivity is measured in a liquid scintillation counter. |
References |
Density | 1.3±0.1 g/cm3 |
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Boiling Point | 706.5±70.0 °C at 760 mmHg |
Molecular Formula | C28H24N4O3 |
Molecular Weight | 464.515 |
Flash Point | 381.1±35.7 °C |
Exact Mass | 464.184845 |
LogP | 5.94 |
Vapour Pressure | 0.0±2.2 mmHg at 25°C |
Index of Refraction | 1.689 |
Storage condition | 2-8℃ |