1338934-20-5
1338934-20-5 structure
- Name: MKC9989
- Chemical Name: MKC9989
- CAS Number: 1338934-20-5
- Molecular Formula: C17H20O7
- Molecular Weight: 336.336
- Catalog: Signaling Pathways Cell Cycle/DNA Damage IRE1
- Create Date: 2018-05-20 14:13:09
- Modify Date: 2024-01-30 19:14:07
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MKC9989 is a Hydroxy aryl aldehydes (HAA) inhibitor and also inhibits IRE1α with an IC50 of 0.23 to 44 μM.
| Name | MKC9989 |
|---|---|
| Synonyms |
2H-1-Benzopyran-8-carboxaldehyde, 7-hydroxy-6-methoxy-3-[2-(2-methoxyethoxy)ethyl]-4-methyl-2-oxo-
7-Hydroxy-6-methoxy-3-[2-(2-methoxyethoxy)ethyl]-4-methyl-2-oxo-2H-chromene-8-carbaldehyde |
| Description | MKC9989 is a Hydroxy aryl aldehydes (HAA) inhibitor and also inhibits IRE1α with an IC50 of 0.23 to 44 μM. |
|---|---|
| Related Catalog | |
| Target |
IC50: 0.23 to 44 μM (IRE1α)[1] |
| In Vitro | At 10 μM concentration, MKC9989 completely inhibits both basal and thapsigargin induced splicing of XBP1 mRNA. These effects are observed even in cells pre-treated with thapsigargin, indicating that MKC9989 can fully reverse the onset of XPB1 splicing after the UPR is initiated. In parallel analysis, MKC9989, significantly stabilizes the RIDD target CD59 mRNA when co-administered with thapsigargin relative to thapsigargin treatment alone and modestly increases levels of CD59 mRNA in non-stressed cells, the latter likely reflects the inhibition of baseline RIDD activity. In contrast to effects on XBP1 splicing, MKC9989 moderately stabilizes CD59 levels when administered 2 hour post treatment with thapsigargin. Finally, the potency of MKC9989 against the splicing of XBP1 mRNA (EC50=0.33 μM) is comparable to its potency against RNA cleavage in vitro[1]. |
| Cell Assay | Human RPMI 8226 plasmacytoma cells are grown in monolayer culture using medium (DMEM) supplemented with 10% fetal calf serum (FCS) at 37°C and 5% CO2. MKC9989 is prepared as 10 mM stocks in DMSO, stored at -20°C, and diluted in medium. Thapsigargin (Tg) is resuspended in DMSO and diluted in medium. Cells are grown to 50% confluency, treated with 1 μM Tg and/or 10 μM MKC9989 at the indicated time points. After incubation of cells for the indicated periods, cells are harvested[1]. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 512.2±50.0 °C at 760 mmHg |
| Molecular Formula | C17H20O7 |
| Molecular Weight | 336.336 |
| Flash Point | 184.5±23.6 °C |
| Exact Mass | 336.120911 |
| LogP | 2.18 |
| Vapour Pressure | 0.0±1.4 mmHg at 25°C |
| Index of Refraction | 1.561 |
| Storage condition | 2-8℃ |