56563-17-8

56563-17-8 structure

Name: PluriSIn #2
Chemical Name: 1-phenylcarbamoyl-5-fluorouracil
CAS Number: 56563-17-8
Molecular Formula: C₁₁H₈FN₃O₃
Molecular Weight: 249.19800
Catalog: Signaling Pathways Cell Cycle/DNA Damage Topoisomerase
Create Date: 2018-06-22 18:28:48
Modify Date: 2024-01-10 22:19:44
PluriSIn #2 is a selective transcriptional inhibitor of topoisomerase II α (TOP2A). PluriSIn #2 is a compound that selectively eliminates undifferentiated human pluripotent stem cells (hPSCs)[1].

Name	1-phenylcarbamoyl-5-fluorouracil
Synonyms	5-Fluor-1-phenylcarbamoyl-uracil 1-Phenylcarbamoyl-5-fluoruracil 5-Fluoro-2,4-dioxo-3,4-dihydro-2H-pyrimidine-1-carboxylic acid phenylamide 5-fluoro-2,4-dioxo-3,4-dihydro-2H-pyrimidine-1-carboxylic acid anilide 5-fluoro-1-phenylcarbamoyluracil

Description	PluriSIn #2 is a selective transcriptional inhibitor of topoisomerase II α (TOP2A). PluriSIn #2 is a compound that selectively eliminates undifferentiated human pluripotent stem cells (hPSCs)[1].
Related Catalog	Research Areas >> Cancer Signaling Pathways >> Cell Cycle/DNA Damage >> Topoisomerase
Target	topoisomerase II alpha
In Vitro	PluriSIn #2 (20 µM; 12 hours) treatment reduces TOP2A expression at the protein level[1]. A 24h exposure of PluriSIn #2 (20 µM) is sufficient to induce massive cell death in hPSCs, but doesn’t affect the viability of various other cell types derived from them[1]. Western Blot Analysis[1] Cell Line: B153 and HUES9 human pluripotent stem cells (hPSCs) Concentration: 20 µM Incubation Time: 12 hours Result: Reduced TOP2A expression at the protein level.
References	[1]. Uri Ben-David, et al. Brief reports: Controlling the survival of human pluripotent stem cells by small molecule-based targeting of topoisomerase II alpha. Stem Cells. 2015 Mar;33(3):1013-9.