| In Vitro |
Moroidin (1) has cytotoxicity to cancer cells with IC50 values of 3.2, 8.3, 9.6, 5.2 and 9.9 μM in A549, H1299, U87, U251 and HCT116 cells, respectively. Moroidin (1) interferes with microtubule polymerization and decreases β-tubulin protein levels in A549 cells. Moroidin (1) arrests the cell cycle in the G2/M phase and induces apoptosis in A549 cells. Moroidin (1) induces A549 cellular apoptosis by the intrinsic mitochondrial pathway. Moroidin (1) represses the migration and invasion of A549 cells[1]. Cell Cytotoxicity Assay[1] Cell Line: A549, H1299, U87, U251, HCT116 and MCF-7 cells Concentration: 0-80 μM Incubation Time: 24, 48, or 72 h Result: Inhibited the proliferation of cancer cells in vitro and exhibited the high sensitivity with the low IC50value in A549 cells. Cell Viability Assay[1] Cell Line: A549 cells Concentration: 0.01-100 μM Incubation Time: 24 h, 48 h, 72 h Result: Is not as potent as colchicine in inhibiting tubulin polymerization and proliferation in A549 cells. Apoptosis Analysis[1] Cell Line: A549 cells Concentration: 0-20 μM Incubation Time: 48 h Result: Caused a dose-dependent increase in the proportion of cells in the G2/M phase, a corresponding decrease in the ratio of cells in the G1 phase and increased significantly the percentage of apoptosis. Immunofluorescence[1] Cell Line: A549 cells Concentration: 2.5 μM, 5 μM Incubation Time: 48 h Result: Resulted in a dose-dependent reduction and fragmentation of the microtubule network, accompanied by cell morphology alteration and fluorescence signal attenuation. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 2.5 μM, 5 μM, 10μM Incubation Time: Result: Resulted in a dosedependent decrease in the protein level of tubulin in A549 cells. Cell Migration Assay [1] Cell Line: A549 cells Concentration: 0.5, 1, 2 μM Incubation Time: 0、24、48 h Result: Showed a significant inhibition in migration of A549 cells, the migrated number of A549 cells was reduced significantly in a dosedependent manner. Cell Invasion Assay[1] Cell Line: A549 cells Concentration: 0.5, 1, 2 μM Incubation Time: 0、24、48 h Result: Decreased the invasion number of A549 cells with dose-dependentance.
|
