870142-71-5
870142-71-5 structure
- Name: ACH-806
- Chemical Name: N-[[4-pentoxy-3-(trifluoromethyl)phenyl]carbamothioyl]pyridine-3-carboxamide
- CAS Number: 870142-71-5
- Molecular Formula: C19H20F3N3O2S
- Molecular Weight: 411.44100
- Catalog: Signaling Pathways Anti-infection HCV
- Create Date: 2016-05-19 00:01:42
- Modify Date: 2024-01-18 11:33:31
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ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM.
| Name | N-[[4-pentoxy-3-(trifluoromethyl)phenyl]carbamothioyl]pyridine-3-carboxamide |
|---|---|
| Synonyms |
ach-806
unii-290j0f5t07 gs-9132 |
| Description | ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM. |
|---|---|
| Related Catalog | |
| Target |
NS4A[1] EC50: 14 nM (HCV)[1] |
| In Vitro | ACH-806 is an NS4A antagonist which can inhibit Hepatitis C Virus (HCV) replication with an EC50 of 14 nM. ACH-806 treatment results in significant reductions of both NS3 and NS4A in the transfected cells. This finding is reminiscent of ACH-806-treated replicon cells in which the amounts of NS3 and NS4A are also both decreased. The total amount of NS3 in the ACH-806-treated sample is reduced by ~6-fold (100/16) and causes a reduction of NS4A-bound NS3 ~29-fold (261/9). The levels of labeled NS3 and NS4A immunoprecipitated by anti-NS3 antibody are apparently reduced after the treatment of ACH-806. ACH-806 also induces significant decreases of NS3 and NS4A and promotes p14 formation in the parental replicon cells but not in the ACH-806-resistant replicon cells[1]. |
| Cell Assay | Huh-luc/neo cells are seeded in 96-well plates at a density of 8000 cells per well in a final volume of 200 μL of Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum. One day after seeding, ACH-806 is serially diluted in 100% dimethyl sulfoxide (DMSO) and added to cells at a 1:200 dilution, achieving a final concentration of 0.5% DMSO in a total volume of 200 μL. Cells are further incubated for 3 days (96 h post-seeding), and the inhibition of HCV replicon replication is quantified by measurement of luciferase activity using a commercial kit[1]. |
| References |
| Molecular Formula | C19H20F3N3O2S |
|---|---|
| Molecular Weight | 411.44100 |
| Exact Mass | 411.12300 |
| PSA | 105.87000 |
| LogP | 5.59150 |