315694-89-4
315694-89-4 structure
- Name: TC-DAPK 6
- Chemical Name: (4E)-2-[(E)-2-phenylethenyl]-4-(pyridin-3-ylmethylidene)-1,3-oxazol-5-one
- CAS Number: 315694-89-4
- Molecular Formula: C17H12N2O2
- Molecular Weight: 276.289
- Catalog: Signaling Pathways Apoptosis DAPK
- Create Date: 2016-07-15 07:50:57
- Modify Date: 2025-08-25 19:12:33
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TC-DAPK 6 is a potent, ATP-competitive, and highly selective DAPK inhibitor (IC50=69 and 225 nM against DAPK1 and DAPK3, respectively, with 10 μM ATP).
| Name | (4E)-2-[(E)-2-phenylethenyl]-4-(pyridin-3-ylmethylidene)-1,3-oxazol-5-one |
|---|---|
| Synonyms |
(4Z)-2-[(E)-2-Phenylvinyl]-4-(3-pyridinylmethylene)-1,3-oxazol-5(4H)-one
tc-dapk 6 5(4H)-Oxazolone, 2-[(E)-2-phenylethenyl]-4-(3-pyridinylmethylene)-, (4Z)- cs-1062 |
| Description | TC-DAPK 6 is a potent, ATP-competitive, and highly selective DAPK inhibitor (IC50=69 and 225 nM against DAPK1 and DAPK3, respectively, with 10 μM ATP). |
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| Related Catalog | |
| Target |
IC50: 69 nM (DAPK1), 225 nM (DAPK3)[1] |
| In Vitro | TC-DAPK 6 is found to be the most potent Death-associated protein kinase (DAPK) inhibitor with enzyme selectivity. When assayed with 10 μM ATP, the IC50 values for DAPK1 and DAPK3 are 69 and 225 nM, respectively. TC-DAPK 6 also inhibits p70S6K (1 μM < IC50< 10 μM)[1]. |
| Kinase Assay | Kinase assay is performed using the Z’-LYTE kinase assay kit Ser/Thr 13 peptide. The standard reaction for compound screening contained 1 mM peptide substrate, 10 mM ATP, 50 mM HEPES (pH 7.4), 10 mM MgCl2, 0.01% Brij-35, and 0.5% DMSO. Human recombinant DAPK1 is used at a final concentration of 2.6 μg/mL, and recombinant DAPK3 is used at a final concentration 1.5 mg/mL. To test the enzyme selectively of the inhibitors (e.g., TC-DAPK 6), ProfilerPro kits are used in the protocol[1]. |
| References |
| Density | 1.2±0.1 g/cm3 |
|---|---|
| Boiling Point | 439.6±55.0 °C at 760 mmHg |
| Molecular Formula | C17H12N2O2 |
| Molecular Weight | 276.289 |
| Flash Point | 219.7±31.5 °C |
| Exact Mass | 276.089874 |
| PSA | 51.55000 |
| LogP | 3.62 |
| Vapour Pressure | 0.0±1.1 mmHg at 25°C |
| Index of Refraction | 1.615 |
| Storage condition | 2-8℃ |
| HS Code | 2934999090 |
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| HS Code | 2934999090 |
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| Summary | 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |