Name | tris(2-carboxyethyl)phosphine hydrochloride |
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Synonyms |
Propanoic acid, 3,3',3''-phosphinidynetris-, hydrochloride (1:1)
3-[bis(2-carboxyethyl)phosphanyl]propanoic acid,hydrochloride 3,3',3''-Phosphinetriyltripropanoic acid hydrochloride (1:1) Tris(2-carboxyethyl)phosphine Hydrochloride 3,3',3''-Phosphinetriyltripropanoic acid hydrochloride MFCD00145469 |
Description | TCEP hydrochloride (Tris(2-carboxyethyl)phosphine hydrochloride) is a non-thiol reducing agent that is more stable and produces a faster S-S reductive reaction than other chemical reductants. TCEP hydrochloride is a trialkylphosphine, selectively reduces protein disuldes without altering the properties or interacting with thiol-directed agents in the reaction mixture. TCEP hydrochloride is also a commonly used reducing agent in the DNA/AuNP chemistry[1][2][3][4]. |
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Related Catalog | |
In Vitro | TCEP hydrochloride has been introduced which oers the prospect of serving as an alternative to the more commonly employed DTT in the NF-κB-DNA binding reactions in vitro, using recombinant p50 protein and a 32P-labelled κB oligonucleotide. DTT promotes NF-κB-DNA binding in concentrations from 0.25 to 2.6 mM in binding reactions. However, in the presence of 0.25 mM DTT, inhibition of NF-κB binding is seen only at Hg2+ concentrations greater than 100 μM and results are highly variable. In contrast, TCEP hydrochloride promotes NF-κB-DNA binding in a dose-related manner in concentrations from 0.25 to 6 mM. In the presence of even 6 mM TCEP hydrochloride, Hg2+ prevents NF-κB-DNA binding at concentrations as low as 20 μM in binding reactions[1]. The human lactoferrin (hLF) peptide is dissolved in phosphate buffer to a concentration of 0.1 mm. Reduction of the disulfide bonds is obtained by adding a 30-fold molar excess of TCEP hydrochloride with subsequent incubation for 2 h at 37 ℃[2]. |
References |
Density | 1.041 g/mL at 25 °C |
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Boiling Point | 519.4ºC at 760 mmHg |
Melting Point | 177 °C |
Molecular Formula | C9H16ClO6P |
Molecular Weight | 286.647 |
Flash Point | 267.9ºC |
Exact Mass | 286.037292 |
PSA | 125.49000 |
LogP | 1.69440 |
Appearance | powder | white |
Index of Refraction | n20/D 1.367 |
Storage condition | 2-8°C |
Water Solubility | H2O: 50 mg/mL |
Symbol |
GHS05 |
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Signal Word | Danger |
Hazard Statements | H314 |
Precautionary Statements | P280-P305 + P351 + P338-P310 |
Personal Protective Equipment | Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges |
Hazard Codes | Xi:Irritant; |
Risk Phrases | R36/37/38 |
Safety Phrases | S26-S37/39-S36/37/39-S27 |
RIDADR | UN 3261 8/PG 2 |
WGK Germany | 2 |
Packaging Group | III |
Hazard Class | 8.0 |
HS Code | 2915900090 |
HS Code | 2915900090 |
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Summary | 2915900090 other saturated acyclic monocarboxylic acids and their anhydrides, halides, peroxides and peroxyacids; their halogenated, sulphonated, nitrated or nitrosated derivatives VAT:17.0% Tax rebate rate:9.0% Supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward) MFN tariff:5.5% General tariff:30.0% |