Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770 (667-675)-Lys(Dnp) ammonium acetate salt

Names

[ CAS No. ]:
1802078-31-4

[ Name ]:
Mca-(Asn670,Leu671)-Amyloid β/A4 Protein Precursor770 (667-675)-Lys(Dnp) ammonium acetate salt

[Synonym ]:
L-Lysine, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-seryl-L-α-glutamyl-L-valyl-L-asparaginyl-L-leucyl-L-α-aspartyl-L-alanyl-L-α-glutamyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)-
N-[(7-Methoxy-2-oxo-2H-chromen-4-yl)acetyl]-L-seryl-L-α-glutamyl-L-valyl-L-asparaginyl-L-leucyl-L-α-aspartyl-L-alanyl-L-α-glutamyl-L-phenylalanyl-N6-(2,4-dinitrophenyl)-L-lysine

Biological Activity

[Description]:

Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.

[Related Catalog]:

Signaling Pathways >> Neuronal Signaling >> Beta-secretase

Research Areas >> Others

[References]

[1]. Vila-Real H, et al. Peptidomimetic β-Secretase Inhibitors Comprising a Sequence of Amyloid-β Peptide for Alzheimer's Disease. J Med Chem. 2015 Jul 23;58(14):5408-18.

[2]. Yen YC, et al. Development of an Efficient Enzyme Production and Structure-Based Discovery Platform for BACE1 Inhibitors. Biochemistry. 2019 Nov 5;58(44):4424-4435.

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Boiling Point ]:
1881.2±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C₆₈H₈₈N₁₄O₂₇

[ Molecular Weight ]:
1533.50

[ Flash Point ]:
1091.5±34.3 °C

[ Exact Mass ]:
1532.594360

[ LogP ]:
3.47

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.600