Resazurin sodium salt

Names

[ Name ]:
Resazurin sodium salt

[Synonym ]:
3H-Phenoxazin-3-one, 7-hydroxy-, 10-oxide, sodium salt (1:1)
7-hydroxy-10-oxy-phenoxazin-3-one,sodium-compound
ResazurinGr
EINECS 263-718-5
7-sodiooxy-3H-phenoxazin-3-one 10-oxide
DIAZORESORCINOL
RESAZURIN SODIUM
7-hydroxy-3H-phenoxazin-3-one 10-oxide sodium salt
Sodium 3-oxo-3H-phenoxazin-7-olate 10-oxide
Resazurine
RESAZOIN
REZAZURIN
RESAZURIN NA-SALT
SODIUM RESAZURIN
REISHIMUSHROOM.P.E
7-Hydroxy-10-oxy-phenoxazin-3-on,Natrium-Verbindung
MFCD00005036

Biological Activity

[Description]:

Resazurin sodium salt is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Biochemical Assay Reagents

Research Areas >> Others

[In Vitro]

Resazurin sodium salt is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin. No viable bacteria are detected 24 h post-inoculation following inclusion of Resazurin sodium salt in TSBc cultures of F. tularensis LVS at the concentration of 44 μM. Lowering the Resazurin sodium salt concentration to as little as 4.4 μM still results in a 10-fold reduction in viable F. tularensis LVS compare to growth medium alone. Both Resazurin sodium salt treatments result in a significant decrease in viable F. tularensis LVS bacteria over 22 h. Treatment with Resazurin sodium salt significantly reduces the number of viable F. tularensis LVS bacteria in HEK293 cells 22 h post-infection[1].

[Cell Assay]

F. tularensis is cultured in TSBc supplemented with 44 μM Resazurin sodium salt at 37°C with shaking for 24 h. At select time points, a Spectronic 200 Spectrophotometer is used to measure the absorbance at 600 nm and 570 nm to detect the presence of Resazurin sodium salt and resorufin, respectively. The ratio of these two optical densities is used to evaluate reduction of Resazurin sodium salt to resorufin over time[1].

[References]

[1]. Schmitt DM, et al. The use of resazurin as a novel antimicrobial agent against Francisella tularensis. Front Cell Infect Microbiol. 2013 Dec 6;3:93.

[Related Small Molecules]

Chemical & Physical Properties

[ Boiling Point ]:
477.2ºC at 760 mmHg

[ Melting Point ]:
>200ºC

[ Molecular Formula ]:
C₁₂H₆NNaO₄

[ Molecular Weight ]:
251.170

[ Flash Point ]:
242.4ºC

[ Exact Mass ]:
251.019455

[ PSA ]:
78.73000

[ LogP ]:
2.47010

[ Storage condition ]:
Store at RT.

[ Stability ]:
Stable. Incompatible with strong oxidizing agents.

[ Water Solubility ]:
soluble

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xn:Harmful;

[ Risk Phrases ]:
R22;R36/37/38

[ Safety Phrases ]:
S26-S36/37/39

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
2934999090

Customs

[ HS Code ]: 2934999090

[ Summary ]:
2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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