Ciprofloxacin Lactate Soluble

Ciprofloxacin (Bay-09867) lactate is a potent, orally active topoisomerase IV inhibitor. Ciprofloxacin lactate induces mitochondrial DNA and nuclear DNA damage and lead to mitochondrial dysfunction, ROS production. Ciprofloxacin lactate has anti-proliferative activity and induces apoptosis. Ciprofloxacin lactate is a fluoroquinolone antibiotic, exhibiting potent antibacterial activity[1][2][3][4].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Cell Cycle/DNA Damage >> Topoisomerase

Research Areas >> Infection

Signaling Pathways >> Anti-infection >> Bacterial

Ciprofloxacin (Bay-09867) lactate (5-50 μg/mL; 0-24 h; tendon cells) inhibits cell proliferation and causes cell cycle arrest at the G2/M phase[1]. Ciprofloxacin (Bay-09867) lactate shows potent activity against Y. pestis and B. anthracis with MIC90 of 0.03 μg/mL and 0.12 μg/mL, respectively[2]. Cell Cycle Analysis[1] Cell Line: Tendon cells Concentration: 5, 10, 20 and 50 μg/mL Incubation Time: 24 hours Result: Decreased the cellularity of tendon cells. Apoptosis Analysis[1] Cell Line: Tendon cells Concentration: 50 μg/mL Incubation Time: 24 hours Result: Arrested cell cycle at the G2/M phase and inhibited cell division in tendon cells. Western Blot Analysis[1] Cell Line: Tendon cells Concentration: 50 μg/mL Incubation Time: 0, 6, 12, 17 and 24 hours Result: Down-regulated the expression of CDK-1 and cyclin B protein and mRNA. Up-regulated the expression of PLK-1 protein.

Ciprofloxacin (Bay-09867) lactate (30 mg/kg; i.p.; for 24 hours; BALB/c mice) has protection against Y. pestis in murine model of pneumonic plague[3]. Ciprofloxacin (Bay-09867) lactate (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) accelerates aortic root enlargement and increases the incidence of aortic dissection and rupture by decreases LOX level and increases MMP levels and activity in the aortic wall[4]. Ciprofloxacin (Bay-09867) lactate (100 mg/kg; i.g.; daily, for 4 weeks; C57BL/6J mice) induces DNA damage and release of DNA to the cytosol, mitochondrial dysfunction, and activation of cytosolic DNA sensor signaling. Ciprofloxacin lactate increases apoptosis and necroptosis in the aortic wall[4]. Animal Model: BALB/c mice[3] Dosage: 30 mg/kg Administration: Intraperitoneal injection; for 24 hours Result: Reduced the lung bacterial load in murine model of pneumonic plague. Animal Model: C57BL/6J mice[4] Dosage: 100 mg/kg Administration: Oral gavage; daily, for 4 weeks Result: Had aortic destruction that was accompanied by decreased LOX expression and increased MMP expression and activity. Animal Model: C57BL/6J mice[4] Dosage: 100 mg/kg Administration: Oral gavage; daily, for 4 weeks Result: Caused mitochondrial DNA and nuclear DNA damage, leading to mitochondrial dysfunction and ROS production. Increased apoptosis and necroptosis in the aortic wall.

[1]. Tsai WC, et, al. Ciprofloxacin-mediated cell proliferation inhibition and G2/M cell cycle arrest in rat tendon cells. Arthritis Rheum. 2008 Jun;58(6):1657-63.

[2]. Steenbergen J, et, al. In Vitro and In Vivo Activity of Omadacycline against Two Biothreat Pathogens, Bacillus anthracis and Yersinia pestis. Antimicrob Agents Chemother. 2017 Apr 24;61(5):e02434-16.

[3]. Hamblin KA, et, al. Inhaled Liposomal Ciprofloxacin Protects against a Lethal Infection in a Murine Model of Pneumonic Plague. Front Microbiol. 2017 Feb 6;8:91.

[4]. LeMaire SA, et, al. Effect of Ciprofloxacin on Susceptibility to Aortic Dissection and Rupture in Mice. JAMA Surg. 2018 Sep 1;153(9):e181804.