Neocarzinostatin

Names

[ Name ]:
Neocarzinostatin

Biological Activity

[Description]:

Neocarzinostatin, a potent DNA-damaging, anti-tumor antibiotic, recognizes double-stranded DNA bulge and induces DNA double strand breaks (DSBs). Neocarzinostatin induces apoptosis. Neocarzinostatin has potential for EpCAM-positive cancers treatment [1][2].

[Related Catalog]:

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis

[In Vitro]

The EpCAM aptamer conjugated Neocarzinostatin (NCS) shows specificity to EpCAM-positive cells. The effects of the conjugates on cancer cells are impressive as the conjugate arrests the cell cycle and promoted apoptosis and necrosis. The high levels of PARP expression confirmed the DNA breaks upon conjugate treatment. NCS conjugated with EpCAM can be targeted to cancer cells sparing normal cells[3]. The IC50 values of Neocarzinostatin (72 hours) to C6 cells and U87MG cells were 493.64 nM, 462.96 nM, respectively[4]. In human tumor cell lines of different tissue origins, sensitivity to neocarzinostatin is proportional to the product of the relative contents of Bcl-2 and caspase-3[5].

[References]

[1]. Athyala PK, et al. Probing the biophysical interaction between Neocarzinostatin toxin and EpCAM RNA aptamer. Biochem Biophys Res Commun. 2016 Jan 8;469(2):257-62.

[2]. Allen Taylor, et al. Ubiquitination capabilities in response to neocarzinostatin and H2O2 stress in cell lines from patients with ataxia-telangiectasia. Oncogene (2002) 21, 4363- 4373.

[3]. Athyala PK, et al. Neocarzinostatin, Aptamer Conjugates for Targeting EpCAM-positive Tumor Cells. Anticancer Res. 2017 Jul;37(7):3615-3629.

[4]. Tianqin G, et al. Synergistic Anti-glioma Effects in Vitro and in Vivo of Enediyne Antibiotic Neocarzinostatin and Paclitaxel via Enhanced Growth Delay and Apoptosis-Induction. Biol Pharm Bull. 2016 Oct 1;39(10):1623-1630.

[5]. Rogers D, et al. Molecular predictors of human nervous system cancer responsiveness to enediyne chemotherapy.

Chemical & Physical Properties

[ Density]:
1.52g/cm3

[ Boiling Point ]:
900.5ºC at 760 mmHg

[ Molecular Formula ]:
C₃₅H₃₅NO₁₂

[ Molecular Weight ]:
661.65200

[ Flash Point ]:
498.4ºC

[ Exact Mass ]:
661.21600

[ PSA ]:
174.77000

[ LogP ]:
1.85820

[ Index of Refraction ]:
1.688

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)

[ RIDADR ]:
NONH for all modes of transport

Articles

Neocarzinostatin-chromophore: a potent inhibitor of casein kinase II in vitro. Tanoue, S., et al.

Antibiotics 51 , 95-98, (1998)

The enediyne antibiotics.

J. Med. Chem. 39 , 2103-2117, (1996)

Reinvestigation of the proteolytic activity of neocarzinostatin.

J. Bacteriol. 182 , 1812-1818, (2000)

Neocarzinostatin (NCS) is the most studied member of a family of chromoproteins secreted by a range of actinomycetes species. It has been proposed that in addition to their antitumoral activity relate...

Related Compounds

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