AM 1042

Asukamycin, a manumycin-type metabolite, could be isolated from Streptomyces nodosus subsp. asukaensis. Asukamycin is an Antibiotic and has antimicrobial activity. Asukamycin inhibits growth of various tumor cell lines[1][2].

Research Areas >> Cancer

Research Areas >> Infection

Signaling Pathways >> Anti-infection >> Fungal

Signaling Pathways >> Anti-infection >> Bacterial

Asukamycin (1-50 μM; 24 h) 以浓度依赖的方式降低五种不同人类细胞系的活力，其 IC50 值为 1-5 μM[1]。 Asukamycin (50 μM; 24 and 72 h; THP-1 细胞) 导致细胞内 caspase 8 和 caspase 3 活性显著增加[1]。 Asukamycin (0-100 mcg/mL; 细菌, 18 h; 真菌, 72 h) 具有抗菌活性。Asukamycin 在 0.78-12.5 mcg/mL 的浓度范围内抑制革兰氏阳性菌的生长，在 25 mcg/mL 的浓度范围内抑制 Trichophyton mentagrophytes 的生长。 Cell Viability Assay[1] Cell Line: Human myeloid THP-1, HL-60, U-118 MG, U-87 MG and U-937 cells Concentration: 1-50 μM Incubation Time: 24 hours Result: Reduced viability of five different cell lines in a concentration-dependent manner.

Asukamycin (0-450 mg/kg; i.p.; 小鼠) 体内毒性低。Asukamycin 具有急性毒性，腹腔注射时 LD50 值为 48.5 mg/kg[2]。 Animal Model: Mice[2] Dosage: 0-450 mg/kg Administration: Intraperitoneal injection Result: Had no effect on mice when administered by 450 mg/kg and the acute toxicity (LD50) was 48.5 mg/kg by intraperitoneal injection.

[1]. Shipley PR, et, al. Antitumor activity of asukamycin, a secondary metabolite from the actinomycete bacterium Streptomyces nodosus subspecies asukaensis. Int J Mol Med. 2009 Nov;24(5):711-5.  

[2]. Omura S, et, al. A new antibiotic,, asukamycin, produced by Streptomyces. J Antibiot (Tokyo). 1976 Sep;29(9):876-81.