4-Nitrophenyl β-D-Cellobioside

Names

[ Name ]:
4-Nitrophenyl β-D-Cellobioside

[Synonym ]:
4-NITROPHENYL-β-D-CELLOBIOSIDE
4-Nitrophenyl β-D-Cellobioside
4-NITROPHENYL-BETA-D-CELLOBIOSIDE
4-Nitrophenyl beta-D-Cellobioside
MFCD00069845

Biological Activity

[Description]:

4-Nitrophenyl β-D-Cellobioside (p-Nitrophenyl β-D-cellobioside) is a cellotriose analog, a chromogenic substrate for the detection of cellulase activity. Exoglucanases, endoglucanases, and β-glucosidases hydrolyze 4-Nitrophenyl β-D-Cellobioside to yield p-nitrophenol (PNP)[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Coleman DJ, et al. A long-wavelength fluorescent substrate for continuous fluorometric determination of cellulase activity: resorufin-beta-D-cellobioside. Anal Biochem. 2007 Dec 15;371(2):146-53.

[2]. Yukihiko Hayashi, et al. Transcellobiosylation Reactions Catalyzed by Different Exoglucanase Components of a T richoderma viride Cellulase in Aqueous Organic Solvent. Biocatal Biotransfor, 2003, 21(1): 25-31.

Chemical & Physical Properties

[ Density]:
1.7 g/cm3

[ Boiling Point ]:
795.6ºC at 760 mmHg

[ Melting Point ]:
249-250ºC

[ Molecular Formula ]:
C₁₈H₂₅NO₁₃

[ Molecular Weight ]:
463.39000

[ Flash Point ]:
435ºC

[ Exact Mass ]:
463.13300

[ PSA ]:
224.35000

[ Index of Refraction ]:
1.67

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
methanol: water (2:3): 50 mg/mL, clear, faintly yellow

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
29400090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Articles

Mechanistic studies of active site mutants of Thermomonospora fusca endocellulase E2.

Biochemistry 38(30) , 9746-51, (1999)

Endocellulase E2 from the thermophilic bacterium Thermomonospora fusca is a member of glycosyl-hydrolase family 6 and is active from pH 4 to 10. Enzymes in this family hydrolyze beta-1,4-glycosidic bo...

Structural changes of cellobiohydrolase I (1,4-beta-D-glucan-cellobiohydrolase I, CBHI) and PNPC (p-nitrophenyl-beta-D-cellobioside) during the binding process.

Sci. China,. C, Life Sci. 51(5) , 459-69, (2008)

Conformational changes to 1,4-beta-D-glucan cellobiohydrolase I (CBHI) in response to its binding with p-nitrophenyl beta-D-cellobioside (PNPC) were analyzed by second-derivative fluorescence spectrom...

The competitive inhibition of Trichoderma reesei C30 cellobiohydrolase I by guanidine hydrochloride.

FEBS Lett. 270(1-2) , 143-6, (1990)

The p-nitrophenylcellobiosidase (PNPCase) activity of Trichoderma reesei cellobiohydrolase I (CBH I) was competitively inhibited by concentrations of guanidine hydrochloride (Gdn HCl) that did not aff...