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Ac-Val-Glu-Ile-Asp-AMC

Names

[ CAS No. ]:
219137-97-0

[ Name ]:
Ac-Val-Glu-Ile-Asp-AMC

[Synonym ]:
L-α-Asparagine, N-acetyl-L-valyl-L-α-glutamyl-L-isoleucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-
Ac-Val-Glu-IIe-Asp-7-amino-4-methylcoumarin
N-Acetyl-L-valyl-L-α-glutamyl-L-isoleucyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)-L-α-asparagine
AC-VAL-GLU-ILE-ASP-MCA
AC-VEID-MCA
PASE-6 SUBSTRATE I,FLUOROGENIC
AC-VAL-GLU-ILE-ASP-AMC
N-ACETYL-VAL-GLU-ILE-ASP-7-AMINO-4-METHYLCOUMARIN AMC
AC-VAL-GLU-ILE-ASP-7-AMINO-4-METHYLCOUMARIN

Biological Activity

[Description]:

Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].

[Related Catalog]:

Research Areas >> Others
Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Enzyme activity (rabbit osteoclasts for example)[1]: 1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell). 2. Put treated cells into 4.5 mL lysis buffer. 3. Each lysate is divided into thirds, then incubate with 40 µM Ac-VEID-AMC at 37°C for 1 h. 4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)

Chemical & Physical Properties

[ Density]:
1.3±0.1 g/cm3

[ Boiling Point ]:
1106.8±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C32H43N5O11

[ Molecular Weight ]:
673.711

[ Flash Point ]:
623.2±34.3 °C

[ Exact Mass ]:
673.295898

[ PSA ]:
250.31000

[ LogP ]:
2.36

[ Appearance of Characters ]:
powder | white

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.571

[ Storage condition ]:
−20°C

Related Compounds