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Ac-Leu-Glu-Thr-Asp-AFC

Names

[ CAS No. ]:
210345-02-1

[ Name ]:
Ac-Leu-Glu-Thr-Asp-AFC

[Synonym ]:
Ac-Leu-Glu-Thr-Asp-7-Amino-4-trifluoromethylcoumarin
AFC157
Ac-LGlutD-AFC
Ac-Leu-Glu-Thr-Asp-AFC
L-α-Asparagine, N-acetyl-L-leucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-
N-Acetyl-L-leucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine

Biological Activity

[Description]:

Ac-LETD-AFC is a caspase-8 fluorogenic substrate. Ac-LETD-AFC can measure caspase-8 fluorogenic activity and can be used for the research of cancer cell apoptosis and oxidative stress metabolism[1].

[Related Catalog]:

Research Areas >> Cancer
Signaling Pathways >> Others >> Others
Research Areas >> Metabolic Disease

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Caspase activity assay[1]: 1. Incubate the cells according to your normal protocol. 2. Wash cells once with PBS and centrifugate. 3. Resuspend cells in PBS at a concentration of 1× 107 cells/mL. 4. Prepare the standard reaction buffer which includ 100 μM caspase-8 substrate AC-LETD-AFC. Prepare correspounding standard reaction buffer according to your protocol. 5. Add 15 ul of the cells suspension to a microplate, and mixed with the appropriate peptide substrate dissolved in a standard reaction buffer. 6. Measure substrate cleavage with a microplate reader with excitation wavelength of 360 nm and emission at 460 nm.

Chemical & Physical Properties

[ Density]:
1.4±0.1 g/cm3

[ Boiling Point ]:
1113.8±65.0 °C at 760 mmHg

[ Molecular Formula ]:
C31H38F3N5O12

[ Molecular Weight ]:
729.655

[ Flash Point ]:
627.4±34.3 °C

[ Exact Mass ]:
729.246887

[ PSA ]:
270.54000

[ LogP ]:
1.76

[ Vapour Pressure ]:
0.0±0.3 mmHg at 25°C

[ Index of Refraction ]:
1.567

Related Compounds