ROX-SE

Names

[ CAS No. ]:
209734-74-7

[ Name ]:
ROX-SE

[Synonym ]:
5-carboxy-X-rhodamine succinimidyl ester
5-ROX,SE [5-Carboxy-X-rhodamine,succinimidyl ester]

Biological Activity

[Description]:

5-Carboxy-X-rhodamin N-succinimidyl ester is a labeling reagent. 5-Carboxy-X-rhodamin N-succinimidyl ester can be used in the preparation of charge-modified dye-labeled Dideoxynucleotide Triphosphates (ddNTP) to "direct-load" DNA sequencing[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Finn PJ, Sun L, Nampalli S, Xiao H, Nelson JR, Mamone JA, Grossmann G, Flick PK, Fuller CW, Kumar S. Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing. Nucleic Acids Res. 2002 Jul 1;30(13):2877-85.

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₇H₃₃N₃O₇

[ Molecular Weight ]:
631.67400

[ Exact Mass ]:
631.23200

[ PSA ]:
123.43000

[ LogP ]:
4.58390

[ Storage condition ]:
-20°C

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
26-36

[ RIDADR ]:
NONH for all modes of transport

Precursor & DownStream

Precursor

DownStream

Articles

High-resolution liquid chromatography of fluorescent dye-labeled nucleic acids.

Anal. Biochem. 223 , 39, (1994)

Using 100 mM of triethylammonium acetate as ion-pairing reagent, phosphodiester oligonucleotides labeled fluorescently at their 5' terminus could be separated successfully on alkylated nonporous 2.3-m...

DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.

Nucleic Acids Res. 20 , 2471, (1992)

The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP...

Synthesis and application of charge-modified dye-labeled dideoxynucleoside-5'-triphosphates to 'direct-load' DNA sequencing.

Nucleic Acids Res. 30 , 2877, (2002)

A novel series of charge-modified, dye-labeled 2',3'-dideoxynucleoside-triphosphate terminators were synthesized and evaluated as reagents for DNA sequencing. These terminators possess an advantage ov...

Related Compounds

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