DHODH-IN-1

DHODH-IN-1 (compound 18d) is a potent Dihydroorotate Dehydrogenase (DHODH) inhibitor with an IC50 of 25 nM. DHODH-IN-1 is an inhibitor of pyrimidine biosynthesis pathway[1].

Research Areas >> Infection

Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis

Signaling Pathways >> Metabolic Enzyme/Protease >> Lactate Dehydrogenase

IC50: 25 nM (DHODH)[1]

DHODH-IN-1 (compound 18d) strongly inhibits Jurkat cells proliferation (IC50=0.02 μM)[1]. DHODH-IN-1 (4, 20, 100 nM) blocks the measles virus replication in cells[1]. DHODH-IN-1 (0.008, 0.04, 0.2, 1, 5 μM) increases the expression of an ISRE-luciferase reporter gene when transfecting cells with ssRNA molecules[1]. Cell Proliferation Assay[1] Cell Line: Jurkat cells Concentration: 0.00001, 0.0001, 0.001, 0.01, 0.1, 1, 10 μM Incubation Time: 72 hours Result: Strongly inhibited Jurkat cells proliferation (IC50=0.02 μM).

DHODH-IN-1 (compound 18d) has a t1/2 of 27–41 min[1].

[1]. Lucas-Hourani M, et al.Original 2-(3-Alkoxy-1H-pyrazol-1-yl)azines Inhibitors of Human Dihydroorotate Dehydrogenase (DHODH).J Med Chem. 2015 Jul 23;58(14):5579-98.