MQAE

Names

[ Name ]:
MQAE

[Synonym ]:
(6-Methoxyquinolinio)acetic acid ethyl ester bromide,MQAE
(6-Methoxyquinolinio)acetic acid ethyl ester bromide MQAE
ethyl 2-(6-methoxyquinolin-1-ium-1-yl)acetate,bromide
MQAE

Biological Activity

[Description]:

MQAE is a fluorescent indicator that is quenched via collision with chloride, and is more sensitive and selective than 36Cl and microelectrode-based methods for chloride measurement in cells.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Bath-applied to acute brain slices, MQAE provides high-quality labeling of neuronal cells and their processes[1]. MQAE fluorescence is adequate and comparable method for measuring cAMP-dependent chloride transport in individual cells[2]. MQAE can be used to measure intracellular chloride concentration in primary cultures of rat aortic smooth muscle cells (VSMC)[3].

[References]

[1]. Kovalchuk Y, et al. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85.

[2]. Andersson C, et al. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2002 Dec 15;59(6):531-5.

[3]. Koncz C, et al. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23.

[Related Small Molecules]

Chemical & Physical Properties

[ Melting Point ]:
177-179ºC(lit.)

[ Molecular Formula ]:
C₁₄H₁₆BrNO₃

[ Molecular Weight ]:
326.18600

[ Exact Mass ]:
325.03100

[ PSA ]:
39.41000

[ Storage condition ]:
-20℃

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi

[ RIDADR ]:
NONH for all modes of transport

[ HS Code ]:
2933499090

Customs

[ HS Code ]: 2933499090

[ Summary ]:
2933499090. other compounds containing in the structure a quinoline or isoquinoline ring-system (whether or not hydrogenated), not further fused. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Articles

Synthesis and characterization of improved chloride-sensitive fluorescent indicators for biological applications.

Anal. Biochem. 178 , 355, (1989)

A class of N-substituted quinoline compounds has been introduced recently for the fluorescence measurement of Cl concentration in biological preparations. The most Cl-sensitive compound was 6-methoxy-...

Hypoxia inhibits colonic ion transport via activation of AMP kinase.

Ann. Surg. 254 , 957-963, (2011)

Mucosal hypoxia is a common endpoint for many pathological processes including ischemic colitis, colonic obstruction and anastomotic failure. Previous studies suggest that hypoxia modulates colonic mu...

The membrane-bound bile acid receptor TGR5 is localized in the epithelium of human gallbladders.

Hepatology 50 , 861-870, (2009)

TGR5 (Gpbar-1) is a plasma membrane-bound, G protein-coupled receptor for bile acids. TGR5 messenger RNA (mRNA) has been detected in many tissues, including rat cholangiocytes and mouse gallbladder. A...