Oenothein B

Oenothein B is a dimeric macrocyclic ellagitannin and has widely pharmacological activities, including antioxidant, anti-inflammatory, antifungal, anti-HCV, and antitumor properties. Oenothein B is a potent and specific inhibitor of poly(ADP-ribose) glycohydrolase[1][2].

Signaling Pathways >> Apoptosis >> Apoptosis

Research Areas >> Cancer

Signaling Pathways >> Anti-infection >> HCV

Research Areas >> Infection

Signaling Pathways >> Anti-infection >> Fungal

Research Areas >> Inflammation/Immunology

Signaling Pathways >> Anti-infection >> Bacterial

Oenothein B (15-45 µM; 12-72 hours) has a dose- and time-dependent inhibition rate effect on A549 cells in the range of 12 hours, 24 hours, 36 hours, 48 hours, 60 hours and 72 hours of exposure[1]. Oenothein B (15-45 µM; 24 hours) effectively inhibits the proliferation of A549 cells by inducing apoptosis and arresting cells at G1 stage[1]. Oenothein B (15-45 µM; 24 hours) not only increases the level of intracellular reactive oxygen species (ROS), but also induces the upregulation of intracellular apoptotic triggers (cleavage caspase-3, PARP, cytochrome c level in the cytosol, Bax)[1]. A549 cells[1]15 µM, 30 µM and 45 µM24 hoursArrested cells in the G1 phase.A549 cells[1]15 µM, 30 µM and 45 µM24 hoursBAX, p53, cytochrome c (cytoplasm) and PARP were unregulated significantly; Anti-apoptotic Bcl-2 was decreased significantly in a concentration-dependent manner. Cell Viability Assay[1] Cell Line: A549 cells Concentration: 15 µM, 30 µM and 45 µM Incubation Time: 12 hours, 24 hours, 36 hours, 48 hours, 60 hours and 72 hours Result: Effectively inhibited the proliferation of A549 cells. Apoptosis Analysis[1] Cell Line: A549 cells Concentration: 15 µM, 30 µM and 45 µM Incubation Time: 24 hours Result: Induced apoptosis in A549 cells. Cell Cycle Analysis[1] Cell Line: A549 cells Concentration: 15 µM, 30 µM and 45 µM Incubation Time: 24 hours Result: Arrested cells in the G1 phase. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 15 µM, 30 µM and 45 µM Incubation Time: 24 hours Result: BAX, p53, cytochrome c (cytoplasm) and PARP were unregulated significantly; Anti-apoptotic Bcl-2 was decreased significantly in a concentration-dependent manner.

Oenothein B (100-300 mg/kg; p.o.) has the ability to reduce neuroinflammation in the brain during systemic inflammation in ICR mice[3].

[1]. Xiaodong Pei, et al. Oenothein B inhibits human non-small cell lung cancer A549 cell proliferation by ROS-mediated PI3K/Akt/NF-κB signaling pathway. Chem Biol Interact. 2019 Jan 25;298:112-120.

[2]. Satoru Tamura, et al. Oenothein B, dimeric hydrolysable tannin inhibiting HCV invasion from Oenothera erythrosepala. J Nat Med. 2019 Jan;73(1):67-75.

[3]. Satoshi Okuyama, et al. Oenothein B suppresses lipopolysaccharide (LPS)-induced inflammation in the mouse brain. Int J Mol Sci. 2013 May 7;14(5):9767-78.