Photoreactivity of 5-iodouracil-containing DNA-Sso7d complex in solution: the protein-induced DNA kink causes intrastrand hydrogen abstraction from the 5-methyl of thymine at the 5' side.
Takanori Oyoshi, Andrew H-J Wang, Hiroshi Sugiyama
Index: J. Am. Chem. Soc. 124(10) , 2086-7, (2002)
Full Text: HTML
Abstract
Photoirradiation of 5-iodouracil-containing DNA, d(GTAAT(I)UAC)(2) with Sso7d protein, possessing significant kink in DNA in the crystal structure induces an unprecedented intrastrand H abstraction at the methyl group of T(5), together with selective photooxidations at Met29 of Sso7d. The reactivity of the deoxyuridin-5-yl radical can be explained by the crystal structure of the d(GTAATTAC)(2)-Sso7d complex, suggesting that the interaction of DNA-Sso7d in solution is substantially similar to its crystal structure.
Related Compounds
Related Articles:
Enzymatic primer-extension with glycerol-nucleoside triphosphates on DNA templates.
2009-01-01
[PLoS ONE 4(3) , e4949, (2009)]
2014-07-01
[Anal. Bioanal. Chem 406(19) , 4585-93, (2014)]
1997-01-01
[Bioconjug. Chem. 8(5) , 757-61, (1997)]
A simple crosslinking method, CLAMP, to map the sites of RNA-contacting domains within a protein.
2008-01-01
[Methods Mol. Biol. 488 , 181-90, (2008)]
Photoreactivity of 5-iodouracil-containing DNA-Sso7d complex.
1999-01-01
[Nucleic Acids Symp. Ser. (42) , 171-2, (1999)]