Nucleotides part LXXX: Synthesis of 3'-O fluorescence labeled thymidine derivatives and their 5'-O-triphosphates.
Tamara I Prykota, Wolfgang Pfleiderer
Index: Nucleosides Nucleotides Nucleic Acids 30(7-8) , 544-51, (2011)
Full Text: HTML
Abstract
A new labeling technique attaching a fluorescent pteridine derivative (3, 5) via a linker onto the 3'-OH group of 5'-O-dimethoxytritylthymidine (7) was developed to lead to the conjugates 8 and 11. After detritylation to give 9 and 12, the final conversion into the corresponding 5'-triphosphates (13, 14), which were isolated as sodium salts, was performed by known methods.
Related Compounds
Related Articles:
1980-11-25
[Nucleic Acids Res. 8(22) , 5473-89, (1980)]
Synthesis of mixed oligodeoxyribonucleotides following the solid phase method.
1982-05-25
[Nucleic Acids Res. 10(10) , 3291-301, (1982)]
Synthesis of 14 C-radiolabeled oligonucleotides with a novel phosphoramidite reagent.
1998-05-05
[Bioorg. Med. Chem. Lett. 8(9) , 1123-6, (1998)]
[Aldrichimica Acta 20 , 52, (1987)]
Oligodeoxynucleotide analogues containing 3'-deoxy-3'-C- threo-hydroxymethylthymidine: Synthesis, hybridization properties and enzymatic stability. Svendsen ML, et al.
[Tetrahedron 49(48) , 11341-52, (1993)]