Further study on the use of uncharged beta-cyclodextrin polymer in capillary electrophoresis: enantiomeric separation of some alpha-hydroxy acids.

S Fanali, Z Aturki

Index: Electrophoresis 16(8) , 1505-9, (1995)

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Abstract

Uncharged beta-cyclodextrin polymer was used as chiral selector for the enantiomeric separation of some alpha-hydroxy acids by capillary electrophoresis. Complexation and enantiomeric resolution of mandelic acid, m-hydroxy and p-hydroxymandelic acid, 3,4-dihydroxymandelic acid, as well as 2- and 3-phenyllactic acid were studied, changing the concentration of the beta-cyclodextrin polymer added to the background electrolyte at different pH in the range of 4.5-7. Furthermore, the effects of the concentration of the background electrolyte, column temperature, and applied voltage on chiral resolution were also examined. The best enantiomeric separations were obtained using a background electrolyte at pH 6 containing 100 mg/mL of beta-cyclodextrin polymer.