Biochemical and Biophysical Research Communications 2013-05-03

UV-dependent production of 25-hydroxyvitamin D2 in the recombinant yeast cells expressing human CYP2R1

Kaori Yasuda, Mariko Endo, Shinichi Ikushiro, Masaki Kamakura, Miho Ohta, Toshiyuki Sakaki

Index: Biochem. Biophys. Res. Commun. 434(2) , 311-5, (2013)

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Abstract

• We produce 25-hydroxyvitamin D in the recombinant yeast expressing human CYP2R1. • Vitamin D2 is produced in yeast from endogenous ergosterol with UV irradiation. • We produce 25-hydroxyvitamin D2 in the recombinant yeast without added substrate.

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