Application of TOOS reagent in enzymatic spectrophotometric determination of hydrogen peroxide
Published date: 2024-12-26 16:40:37
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Name | TOOS | CAS# | 82692-93-1 |
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| Price | $1.7/10g $1.4/50g $1.13/100mg | Purity | 99.0% | |
| Stocking Period |
Inquiry | Stock | In Stock | |
| Product Webpage: | https://www.hbdsbio.com/products/toos-cas-no-82692-93-1.html | |||
| Detail
Product Information
In the fields of biochemistry and medical diagnosis, hydrogen peroxide (H ₂ O ₂) is an important reactive oxygen species molecule, and accurate determination of its concentration is of great significance for monitoring various biochemical reactions, disease diagnosis, and evaluating treatment effectiveness. With the advancement of technology, enzymatic photometry has gradually become the preferred method for detecting hydrogen peroxide due to its high sensitivity and easy operation. In this method, N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS), as a novel Trinder's reagent, has brought new breakthroughs to the determination of hydrogen peroxide with its unique advantages and wide applicability. Innovations in TOOS reagents Although traditional Trinder's reagents have been widely used in hydrogen peroxide determination, they have problems such as poor water solubility, insufficient stability, and susceptibility to environmental interference, which limit their application in certain complex samples or special conditions. In contrast, TOOS reagents have innovated in their chemical structure by introducing specific functional groups, significantly improving their water solubility and enabling more uniform dispersion in solution, reducing errors caused by precipitation. In addition, TOOS also exhibits a wider pH adaptability range, which means that it can maintain stable reaction performance under different biological samples and experimental conditions, which is crucial for improving the accuracy and repeatability of detection. The mechanism of enzymatic photometric determination of hydrogen peroxide Enzymatic photometry is a method for determining the concentration of target molecules based on the color changes produced by enzymatic reactions. In the determination of hydrogen peroxide, peroxidase (such as horseradish peroxidase) is usually used as a catalyst, which can promote the oxidative coupling reaction between hydrogen peroxide and TOOS and another auxiliary substrate (such as 4-aminoantipyrine or 3-methylbenzothiazole sulfone). This reaction produces a dye with a strong color (usually purple or blue), whose color depth is proportional to the concentration of hydrogen peroxide. By measuring the absorbance of this dye, the concentration of hydrogen peroxide can be indirectly calculated. |
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