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Application of TOOS reagent in enzymatic spectrophotometric determination of hydrogen peroxide

Product detail:

Published date: 2025-08-21 23:50:45

CAS number: 82692-93-1

Name: TOOS

Price:
$1.7/10g $1.4/50g $1.13/100mg

Purity: 99.0%

Stocking period: Inquiry

Stock: In Stock

Product Webpage: https://www.hbdsbio.com/products/toos-cas-no-82692-93-1.html

Detail Product Information:
In the fields of biochemistry and medical diagnosis, hydrogen peroxide (H ₂ O ₂) is an important reactive oxygen species molecule, and accurate determination of its concentration is of great significance for monitoring various biochemical reactions, disease diagnosis, and evaluating treatment effectiveness. With the advancement of technology, enzymatic photometry has gradually become the preferred method for detecting hydrogen peroxide due to its high sensitivity and easy operation. In this method, N-ethyl-N - (2-hydroxy-3-sulfopropyl) -3-methylaniline sodium salt (TOOS), as a novel Trinder's reagent, has brought new breakthroughs to the determination of hydrogen peroxide with its unique advantages and wide applicability.
Innovations in TOOS reagents
Although traditional Trinder's reagents have been widely used in hydrogen peroxide determination, they have problems such as poor water solubility, insufficient stability, and susceptibility to environmental interference, which limit their application in certain complex samples or special conditions. In contrast, TOOS reagents have innovated in their chemical structure by introducing specific functional groups, significantly improving their water solubility and enabling more uniform dispersion in solution, reducing errors caused by precipitation. In addition, TOOS also exhibits a wider pH adaptability range, which means that it can maintain stable reaction performance under different biological samples and experimental conditions, which is crucial for improving the accuracy and repeatability of detection.
The mechanism of enzymatic photometric determination of hydrogen peroxide
Enzymatic photometry is a method for determining the concentration of target molecules based on the color changes produced by enzymatic reactions. In the determination of hydrogen peroxide, peroxidase (such as horseradish peroxidase) is usually used as a catalyst, which can promote the oxidative coupling reaction between hydrogen peroxide and TOOS and another auxiliary substrate (such as 4-aminoantipyrine or 3-methylbenzothiazole sulfone). This reaction produces a dye with a strong color (usually purple or blue), whose color depth is proportional to the concentration of hydrogen peroxide. By measuring the absorbance of this dye, the concentration of hydrogen peroxide can be indirectly calculated.

Supplier/Manufacture:

Name: Wuhan Desheng Biochemical Technology Co., Ltd

Chemsrc Level: Unverified

Product #: 2

Tel: 0711-3702650

Address: Gaoxin 3rd Road, Huarong District, Ezhou, Hubei Province

Area: China

Contact: Doris Yang

Contact Phone #: 13297690802

Email: sales5@whdschem.com

Website: https://www.hbdsbio.com/

Major Market: India, Türkiye, Russia

Annual Trade Volume:

Foreign Trade % of Salers:

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Detail introduction:
Xindesheng is a professional company engaged in various fine chemical raw materials, biological buffers, blood collection additives, chromogenic matrices, in vitro diagnostic reagents, chemiluminescence reagents, and cosmetic raw materials. We can also provide customized services for biological reagents. Since its establishment in 2005, we have been pursuing excellence, focusing on research and innovation, and promoting the development of our company in the biological reagent industry. We have a leading research and development and production team for biological reagents in China. With cooperation with multiple domestic university research institutions, Desheng has a huge advantage in customizing the synthesis of biological reagents

Top suppliers:

Name: Shanghai Nianxing Industrial Co., Ltd

Area: China

Price:
$1.7/10g $1.4/50g $1.13/100mg

Contact: Yang

Product Detail: TOOS


Name: Shanghai Jizhi Biochemical Technology Co., Ltd

Area: China

Price:
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Contact: Liu jia

Product Detail: TOOS


Name: Dayang Chem (Hangzhou) Co., Ltd.

Area: China

Price:
$1.7/10g $1.4/50g $1.13/100mg

Contact: Ms Wang

Product Detail: Sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate


Name: Henan Tianfu Chemical Co., Ltd.

Area: China

Price:
$1.7/10g $1.4/50g $1.13/100mg

Contact: Anson

Product Detail: Sodium 3-(N-ethyl-3-methylanilino)-2-hydroxypropanesulfonate


Name: Zhongshan Xingrui Chemical Co. , Ltd.

Area: China

Price:
$1.7/10g $1.4/50g $1.13/100mg

Contact: Wang Jie

Product Detail: TOOS


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