Journal of Chromatography B 2005-10-25

Pseudouridine detection improvement by derivatization with methyl vinyl sulfone and capillary HPLC-mass spectrometry.

Gert Emmerechts, Piet Herdewijn, Jef Rozenski

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 825(2) , 233-8, (2005)

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Abstract

A method is presented for improved detection of pseudouridine in nucleoside mixtures based on the specific derivatization with methyl vinyl sulfone followed by analysis by capillary HPLC-mass spectrometry. Reaction conditions were optimized in order to obtain the best yield and specificity. The method was successfully applied to different nucleoside mixtures.