Bioorganicheskaia khimiia 1989-10-01

[Modification of DNA with dihydrazide of succinic acid for preparation of hybridization probes].

M F Turchinskiĭ, E I Aĭnbinder, V D Knorre, S N Shcherbo

Index: Bioorg. Khim. 15(10) , 1341-5, (1989)

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Abstract

A two-step chemical method of introduction of nonradioactive labels in DNA was proposed. At first step DNA is modified by succinic dihydrazide at pH 5.0 and 95 degrees C, or at pH 4.5 and 37 degrees C in presence of sodium bisulfite. Then FITC or biotin are joined to the hydrazide groups. DNA modified in this way were shown to be effective hybridisation probes.