Chemical Communications 2012-02-01

Membrane labeling and immobilization via copper-free click chemistry.

Heidi E Bostic, Matthew D Smith, Andrei A Poloukhtine, Vladimir V Popik, Michael D Best

Index: Chem. Commun. (Camb.) 48(10) , 1431-3, (2012)

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Abstract

Copper-free click chemistry was employed to derivatize membrane bilayers. This approach uses an azido-lipid conjugate presented on liposomes, which can be labeled in bioorthogonal fashion via cyclooctyne-tagged reagents. An immobilization-based approach using streptavidin-coated microplates was exploited to evaluate membrane derivatization.This journal is © The Royal Society of Chemistry 2012