Journal of the American Chemical Society 2006-09-20

Electron paramagnetic resonance studies of an integral membrane peptide inserted into aligned phospholipid bilayer nanotube arrays.

Ethan S Karp, Johnson J Inbaraj, Mikhail Laryukhin, Gary A Lorigan

Index: J. Am. Chem. Soc. 128(37) , 12070-1, (2006)

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Abstract

This communication reports for the first time the determination of the helical tilt of an integral membrane peptide inserted into aligned phospholipids bilayer nanotube arrays using spin label EPR spectroscopy. Also, we demonstrate herein how the helical tilt of the peptide can be easily calculated using the hyperfine splitting values gleaned from a perpendicularly aligned bilayer in phospholipid bilayer nanotube arrays. EPR spectral simulations were used to verify the method.